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Tools for researching the SARS-CoV-2 virus

 

The COVID-19 public health crisis affects everyone in our global community, and many scientists are working very hard to gain more knowledge about the SARS-CoV-2 virus (previously 2019-nCoV) and develop diagnostic tests, treatments and vaccines. Crucial questions about this virus need to be answered so quick and reliable research tools matter now more than ever. We at BIOKÉ support the fight against COVID-19 by offering a range of high quality products for virus research, from viral purification kits to qPCR plates, together with our reliable technical support.

During this COVID-19 pandemic you can expect the same quality and support from us as before. We have implemented multiple measures to keep our employees and business partners safe, and at the same time to maintain the continuity of supply of our products and support to our customers. We are working closely with our suppliers to identify and mitigate risks in the supply of products. Furthermore we are monitoring the situation and following the guidance of national and international health organizations and governmental authorities around the world.

On this page you will find a selection of our products that can be used for virus research. However we have more products that can be relevant, please let us know if there is anything we can do to help your research.

 


Viral purification products

MACHEREY-NAGEL (MN)

Multiple labs have included MACHEREY-NAGEL's NucleoSpin and NucleoMag kits for isolation of viral RNA into their workflows, among them the two WHO reference laboratories, the Institut Pasteur Paris (France) and the National Institute of Health (Thailand). We are supporting all labs by providing guidelines for sample preparation and nucleic acid extraction with MN kits in compliance with WHO and CDC recommendations. The recommendations include all our NucleoSpin and NucleoMag kits suitable for extraction of viral RNA. MN protocol recommendations for respiratory samples (swabs, sputum, bronchoalveolar lavage). Please note, BIOKÉ distributes MACHEREY-NAGEL in the Netherlands only (click here for other countries)

Products:

  • NucleoSpin® RNA Virus* (740956) > see comments below
  • NucleoSpin® Dx Virus* (CE-IVD) (740895) > see comments below
  • NucleoSpin® Virus (740983)
  • NucleoMag® Pathogen (744210)
  • NucleoMag® Virus (744800)
  • NucleoSpin® 8 / 96 Virus (740451)

*For the following reasons MN recommends to use NucleoSpin® RNA Virus instead of NucleoSpin® Dx Virus for SARS-CoV-2 detection:

  1. The CE-IVD mark only applies for plasma/serum, does not include respiratory samples!
  2. NucleoSpin® Dx Virus kit contains Proteinase K and 1.5 mL Tubes, which are not required for SARS-CoV-2 isolation.

The quality assurance management from MN provided a comparison sheet stating the equivalence of NucleoSpin Dx Virus and NucleoSpin RNA Virus kit components.

MN developed cost- and time-efficient support protocols specified for saliva and respiratory swab samples for dedicated products.
NucleoMag® Virus - Viral nucleic acid isolation from saliva and respiratory swab samples – Enlarged number of extractions (Maximize number of reactions per kit)
NucleoMag® Virus - Viral nucleic acid isolation from saliva and respiratory swab samples – Fast Track (protocol to increase sample throughput with less hands-on time)
Nucleospin® 96 Virus - Viral nucleic acid isolation from saliva and respiratory swab samples – Fast Track (protocol to increase sample throughput)

Downloads

 

Viral nucleic acid isolation from water samples

Many researchers are now starting to use wastewater testing to monitor COVID-19 infection rates. Routine wastewater surveillance might be an efficient, non-invasive tool to detect new COVID-19 infections. For efficient isolation of nucleic acids from water samples it is essential to concentrate viral particles prior to nucleic acid purification.

Methods for sample concentration:
Several methods can be used for sample concentration:

MN recommendations for sample concentration prior to nuclei acid purification

 

Nucleic acid purification from concentrated water samples:
We recommend to either use NucleoMag® DNA/RNA Water or NucleoSpin® RNA Stool for nucleic acid purification from concentrated water samples for the following reasons:

  • Superior PCR inhibitor removal characteristics
  • RNA as well as DNA can be extracted

 

 


New England Biolabs (NEB)

Extraction of RNA is often the first step in viral detection assays and has become an increasingly important laboratory technique in the midst of this global pandemic. Though NEB does not offer a kit specifically designed for viral RNA extraction, the Monarch Total RNA Miniprep Kit has been successfully used to extract RNA from various samples including saliva, buccal swabs, and simulated nasopharyngeal samples.

Monarch® Total RNA Miniprep Kit (NEB #T2010): This comprehensive kit include reagents for extracting RNA from a wide variety of sample types with a fast and user-friendly workflow.

  • Quickly and easily purify up to 100 µg of high quality total RNA (>20 nt)
  • Purified RNA is suitable for downstream reactions, such as RT-qPCR and library prep for next gen sequencing
  • Elute in as little as 30 µl (RNA samples can be further concentrated following purification using our Monarch RNA Cleanup columns or kits)

Protocol for Total RNA Purification from Saliva, Buccal Swabs and Nasopharyngeal Samples
Request a sample

 

 

Other RNA products

MACHEREY-NAGEL

  • NucleoProtect RNA stabilization reagent (740400) for cells and tissues
  • One phase total RNA extraction from all kinds of sample with NucleoZOL (740404)

Please note, BIOKÉ distributes MACHEREY-NAGEL in the Netherlands only

 

 

Rapid Detection of Coronavirus (COVID-19) RNA

New England Biolabs

Virus detection often utilizes nucleic acid amplification to identify the presence of specific sequences in SARS-CoV-2 viral RNA. These methods include RT-qPCR for real time quantitation and loop-mediated isothermal amplification (LAMP) for robust amplification performed at a single reaction temperature.

SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (NEB# E2019) utilizes loop-mediated isothermal amplification for use in the analysis of SARS-CoV-2, the novel coronavirus that causes COVID-19.

  • Colorimetric LAMP enables simple, visual detection (pink-to-yellow) of amplification of SARS-CoV-2 nucleic acid
  • Reduce risk of carryover contamination, with UDG and dUTP included in the master mix
  • Assay targets N and E regions of the SARS-CoV-2 genome, for optimized sensitivity and specificity

Extra information

Preprint: Rapid Molecular Detection of SARS-CoV-2 (COVID-19) Virus RNA Using Colorimetric LAMP
Enhancing Colorimetric LAMP Amplification Speed and Sensitivity with Guanidine Chloride
Additional citations and EUA protocols featuring Colorimetric LAMP kits

 

WarmStart Colorimetric LAMP 2X Master Mix with UDG (NEB# M1804) is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of LAMP and RT-LAMP reactions. The inclusion of dUTP and UDG in the master mix reduces the possibility of carryover contamination between reactions.

  • Easily detect amplification with a simple pink-to-yellow color change
  • Reduce risk of carryover contamination, with UDG and dUTP included in the master mix
  • Set up reactions at room temperature, as WarmStart feature inhibits activity at 25°C

Extra information

Preprint: Rapid Molecular Detection of SARS-CoV-2 (COVID-19) Virus RNA Using Colorimetric LAMP
Enhancing Colorimetric LAMP Amplification Speed and Sensitivity with Guanidine Chloride
Additional citations and EUA protocols featuring Colorimetric LAMP kits


WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA) (NEB #M1800) is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution.The mix contains a visible pH indicator for rapid and easy detection of Loop-Mediated Isothermal Amplification (LAMP) and RT-LAMP reactions.

  • Easily detect amplification with a simple pink-to-yellow color change
  • Set up reactions at room temperature, as WarmStart feature inhibits activity at 25°C
  • Compatible with carryover prevention

Extra information

 

WarmStart LAMP Kit (DNA & RNA) (NEB# E1700) contains a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx for optimal LAMP and RT-LAMP performance. It is compatible with multiple detection methods, including real-time fluorescence detection (when used with included LAMP fluorescent dye), turbidity, and end-point visualization.

Extra information

 

Facilitating Detection of SARS-CoV-2: Precursor Studies with Luna® RT-qPCR and Colorimetric RT-LAMP Reagents

This new application note is presented in the context of the COVID-19 global pandemic caused by the SARS-CoV-2 coronavirus. To help accelerate diagnostics development efforts, we demonstrate the detection of synthetic SARS-CoV-2 viral RNA targets using NEB’s RT-qPCR and colorimetric RT-LAMP reagents. The colorimetric RT-LAMP work is a follow-up on a recent publication by researchers at NEB, in collaboration with researchers at the Wuhan Institute of Virology in China, demonstrating the potential of colorimetric RT-LAMP detection of SARS-CoV-2 viral RNA purified from patient samples.

Download this application note

 

Homogenization of cells and tissues

Syntec International

The Medimachine II can be used for the homogenization of cells and tissues to extract biomolecules for molecular biology or protein biochemistry applications. Medimachine II homogenizator provides high quality results for extraction of molecules for the analysis of chemical residues, virus, bacterias etc.

 


 

qPCR reagents

New England Biolabs

Luna Universal One-Step RT-qPCR kits offer exceptional sensitivity, reproducibility and RT-qPCR performance. Available for probe- or dye-based detection, Luna kits utilize a novel, thermostable WarmStart RT, which increases reaction specificity. Reaction mixes also contain dUTP, which supports carryover prevention.

Relevant Luna products include:

  • Luna Universal Probe One-Step RT-qPCR Kit (NEB #E3006)
  • Luna Probe One-Step RT-qPCR Kit (No ROX) (NEB #E3007)
  • Luna Universal One-Step RT-qPCR Kit (NEB #E3005)
  • Luna Cell Ready Lysis Module (NEB #E3032)

Extra information

 

Request your sample

 

High-throughput qPCR and RT-qPCR Workflows: Enabled by Beckman Coulter Echo Acoustic Liquid Handling and NEB Luna Reagents

Luna qPCR reagents are a great choice for automated high-throughput qPCR and RT-qPCR applications. Highlights from a new application note include:

  • Luna qPCR and One-Step RT-qPCR are fully compatible with Echo-mediated acoustic liquid handling
  • Linear, accurate quantitation, sensitive detection, exceptional reproducibility, and robust room temperature stability during automated workflows
  • Echo-mediated reaction setup enables fast, accurate, high-throughput assembly of qPCR experiments
  • An adapted Luna Cell Ready protocol allows direct lysis of cells in Echo-qualified cell culture source plates for automated, high-throughput workflow

Read this application note

 

qPCR plates

4titude (part of Brooks)

qPCR accuracy matters now more than ever. Our partner 4titude provides high-quality qPCR plates for a wide range of cyclers.

Below you will find a selection of 2-component plates that are available for the Roche Lightcycler:
FrameStar® 384 Well Skirted PCR Plate, Roche Style (4ti-0381)
FrameStar® 384 Well Skirted PCR Plate, Roche Style, Plus qPCR Seal (4ti-0382)
FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style (4ti-0951)
FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, Plus qPCR Seal (4ti-0952)
FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity (4ti-0954)
FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity, Plus qPCR Seal (4ti-0954/0560)

The qPCR seals (4ti-0560) are available in the combi packs and separately.

More plates for the Lightcycler and for other cyclers can be found via the Brooks Plate Wizard


Sequencing products

New England Biolabs

In the COVID-19 pandemic, sequencing is enabling scientists, often in unprecedented collaborations, to make rapid advances in epidemiology and surveillance, basic and clinical research, and diagnostics. A fast-growing number of methods are being developed to address these applications, including targeted SARS-CoV-2 sequencing, host genomic sequencing and metagenomic sequencing.

Products for Oxford Nanopore Technologies® Sequencing: The following reagents are being recommended in a number of third party COVID-19 sequencing protocols, including “PCR tiling of COVID-19 virus” in Oxford Nanopore Technologies’ Nanopore Community, and the ARTIC protocol.

  • Q5® Hot Start High-Fidelity 2X Master Mix (NEB #M0494)
  • Random Primer Mix (NEB #S1330S)
  • Deoxynucleotide (dNTP) Solution Mix (NEB #N0447)
  • NEBNext® Ultra™ II End Repair / dA-Tailing Module (NEB #E7546)
  • NEBNext Ultra II Ligation Module (NEB #E7595)
  • NEBNext Quick Ligation Module (NEB #E6056)

More information on these and other protocols

 

Products for Illumina® Sequencing: Reagents available for sequencing on the Illumina platform for this application include:

  • NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770)
  • NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760)
  • NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645)
  • NEBNext Ultra II RNA First Strand Synthesis Module (NEB #E7771)
  • NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (NEB #E6111)
  • NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB #E7400)
  • LunaScript RT SuperMix Kit (NEB #E3010)

Additional citations featuring NEBNext products

 

 

 

Antibody products for researching the host-immune response to SARS-CoV-2

Cell Signaling Technology (CST)

In support of researchers uncovering mechanisms of the SARS-CoV-2 infection, CST is continually developing new antibodies, kits, and reagents to help end the COVID-19 pandemic. This page will be updated regularly to provide the latest status for key targets. If you do not see your target of interest, please contact CST directly.

Latest releases:
SARS-CoV-2 Spike Protein Serological IgG ELISA Kit #20154
ACE2 (OTI1D6) Mouse mAb #15983

 

Bethyl Laboratories

In response to the rapid spread of the new coronavirus SARS-CoV-2, Bethyl Laboratories has developed ELISA kits for the detection of human IgG and IgM antibodies against the virus in serum or plasma to aid vaccine and other COVID-19 related research. Learn more about these SARS-CoV-2 IgG & IgM ELISA Kits

Also the following reagents from Bethyl Laboratories are relevant to COVID-19 (SARS-CoV-2) seroconversion assay development: Human IgM Antibody (goat) (A80-100A), Human IgM Antibody (rabbit) (A80-101A), Human IgG-Fc Fragment Antibody (goat) (A80-104A), Human IgG-Fc Fragment Antibody (rabbit) (A80-105A), Human IgA Antibody (goat) (A80-102A), and Human IgA Antibody (rabbit) (A80-103A). Check out Bethyl's Bulk antibody program and Bethyl can also partner with you to develop recombinant rabbit monoclonals against novel targets.

 

 

Technical Support

Please let us know if there is anything we can do to help your research

E. techsupport@bioke.com

T. +31 (0)71 7200 220 (option 4)

 

 

How can we support you?

BIOKÉ distributes these products in the Netherlands, Belgium and Luxembourg, and MACHEREY-NAGEL products in the Netherlands only

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