Product Class: Kit

WarmStart® LAMP Kit (DNA & RNA)

Need assistance designing LAMP primers? Use the NEB LAMP Primer Design Tool.

This product is available in a glycerol-free format. Contact us for more information.

Product Introduction

  • Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets
  • Compatible with multiple detection methods
  • WarmStart® technology inhibits activity at room temperature
  • Offered with dUTP and thermolabile UDG for carryover contamination prevention (NEB #E1708, M1708)
  • LAMP Fluorescent Dye (NEB #B1700) component available separately
  • This reagent has been incorporated into Oxford Nanopore’s LamPORE technology, a combination of LAMP with nanopore sequencing, initially implemented to provide a rapid, low-cost and scalable assay for the detection of SARS-CoV-2.
  • Learn more about how RT-LAMP is being used for rapid COVID-19 screening
  • Also available: lyophilized LAMP/RT-LAMP mix
Catalog # Size Concentration
E1700S 100.0 reactions
E1700L 500.0 reactions

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Product Information

Description

The WarmStart LAMP Kit (DNA & RNA) is designed to provide a simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provides rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase. This kit is supplied with the WarmStart LAMP 2X Master Mix, which contains a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized LAMP buffer solution. Both Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase have been engineered for improved performance in LAMP and RT-LAMP reactions. A fluorescent dye is also supplied to enable real-time fluorescence measurement of LAMP. The WarmStart LAMP Kit is compatible with multiple detection methods, including turbidity detection, real-time fluorescence detection (when used with LAMP fluorescent dye) and end-point visualization.

This product is also available in a lyophilized format. The LyoPrime WarmStart® Fluorescent LAMP/RT-LAMP Mix (with UDG) is a lyophilized LAMP mix offering ambient/room temperature storage and shipping and streamlined assay development. To learn about the lyophilization process, please visit www.neb.com/lyoprime.


Figure 1. The WarmStart LAMP Kit (DNA & RNA) is compatible with multiple detection methods


The WarmStart LAMP Kit (DNA & RNA) includes fluorescent dye for real-time fluorescence measurement. Alternately, detection can be accomplished by turbidity detection or a variety of endpoint visualization methods.

For carryover contamination prevention, a version containing dUTP and thermolabile UDG is available: WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG) (NEB #E1708).

Figure 2. The WarmStart LAMP Kit (DNA & RNA) offers speed and robust sensitivity

speed and sensitivity


A RNA target (HMBS2) was amplified from Jurkat total RNA using the WarmStart LAMP Kit and OptiGene Master Mix (ISO-001). Reactions were performed at 65°C for 74 minutes on a real-time thermocycler (Bio-Rad CFX96) in triplicate. Time to result is set as the time at which the fluorescence crossed a threshold of 10% of maximal fluorescence. NEB’s WarmStart LAMP Kit resulted in faster and more robust detection as compared to the OptiGene Master Mix.

Figure 3. The WarmStart LAMP Kit (DNA & RNA) provides fast LAMP/RT-LAMP results

Fast LAMP/RT-LAMP results


Experiments were designed to amplify a DNA target (BRCAb) and a RNA target (HMBS2) by LAMP or RT-LAMP, respectively. A 25 μl reaction containing 10 ng HeLa DNA or 5 ng Jurkat total RNA, 1X Master Mix, 1X LAMP primers and fluorescent dye was incubated on a Bio-Rad CFX98 instrument at 65°C for 75 minutes. Lucigen® OmniAmp® RNA & DNA Kit and OptiGene Isothermal Master Mix (ISO-001 ) were run according to manufacturers’ recommendations.

The WarmStart LAMP Kit (DNA & RNA) enables specific amplification across multiple species/targets

PRIMER SET

DNA TEMPLATE

HeLa 
DNA

Lyme 
DNA

B. malayi 
DNA

E. coli
DNA

C. elegans 
DNA

BRCAb (human)

+

 

 

 

 

CFTR (human)

+

 

 

 

 

LymO (Lyme)

 

+

 

 

 

BmHhal (B. malayi)

 

 

+

 

 

dnaE (E. coli)

 

 

 

+

 

Lec10b (C. elegans)

 

 

 

 

+

Lec6 (C. elegans)

 

 

 

 

+

In each individual reaction, multiple gDNA templates were added along with primers specific to a target in only one species. Reactions were incubated at 65°C for 74 minutes on a thermocycler (Bio-Rad CFX96) in triplicate. Positive results (+) were observed only in the expected species/target combinations, demonstrating high specificity of the WarmStart LAMP reactions.

This product is related to the following categories:
Isothermal Amplification & Strand Displacement Products
This product can be used in the following applications:
Loop-Mediated Isothermal Amplification,
Isothermal Amplification

Kit Components

Kit Components

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Materials Required but not Supplied

  • LAMP Primers (we recommend using NEB LAMP Primer Design Tool)
  • Target Nucleic Acid Samples
  • Molecular Biology Grade H2O
  • Heat block, water bath, real-time turbidimeter or thermocycler (with real-time fluorescence measurement if desired) and instrument-appropriate reaction vessels

References

  1. Notomi, T., et al. (2000). Nucleic Acids Res. 28, PubMedID: E63
  2. Nagamine, K., et al. (2001). Clin. Chem. 47, 1742-1743.
  3. Nagamine, K., Hase, T., Notomi, T. (2002). Mol. Cell Probes. 16, 223-229.
  4. Mori, Y., Nagamine, K., Tomita, N., Notomi, T. (2001). Biochem. Biophys. Res. Commun. 289, 150-154.
  5. Mori, Y., Kitao, M., Tomita, N., Notomi, T. (2004). J. Biochem. Biophys. Methods. 59, 145-157.
  6. Tomita, N., Mori, Y., Kanda, H., Notomi, T. (2008). Nat. Protoc. 3, 877-882.
  7. Tanner, N.A. and Evans, T. C. Jr (2014). Curr. Protoc. Mol. Biol. 105, Unit 15.14.
  8. Hsieh et al. (2014). Chem. Comm. 50, 3747-3749.
  9. Tanner, N.A., Zhang, Y. and Evans, T.C. Jr (2015). Biotechniques. 58, 59-68.

Protocols, Manuals & Usage

Protocols

  1. WarmStart LAMP Kit (DNA & RNA) Protocol (E1700)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Application Notes

FAQs & Troubleshooting

FAQs

  1. What components are included in the WarmStart LAMP Kit (DNA & RNA)?
  2. What is the optimal LAMP/RT-LAMP amplification temperature?
  3. What is the Mg++ concentration in the WarmStart LAMP/RT-LAMP Master Mix?
  4. How do I use the fluorescent dye?
  5. What types of input samples/materials are compatible with the LAMP/RT-LAMP mixes?
  6. How fast should I expect a result?
  7. How do I confirm a positive result?
  8. Can I set up my LAMP/RT-LAMP reactions at room temperature?
  9. Can I use dUTP and UDG for carryover contamination prevention with the WarmStart LAMP Master Mix?
  10. The WarmStart LAMP Master Mix has some precipitation in the tube after thawing, is this normal?
  11. How do I design LAMP Primers?
  12. What is the difference between NEB #E1708: WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG) and NEB #E1700: WarmStart LAMP Kit (DNA & RNA)?
  13. Amplification occurred in my NTC sample(s) following isothermal incubation. What happened?
  14. What detection methods can be used for LAMP/RT-LAMP experiments?
  15. What are the advantages of using standard LAMP (NEB #E1700, M1708, E1708) over pH-based colorimetric LAMP (NEB #M1800, M1804)?
  16. Is a separate incubation step required for RT-LAMP?
  17. Does NEB have a master mix for LAMP or RT-LAMP reactions?
  18. What is LAMP and RT-LAMP?
  19. What type of purification is recommended for LAMP primers?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specification Change Notifications

The M1700 component is now supplied in Sarstedt vials (February 28, 2022).

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased product to perform loop-mediated amplification (“LAMP”) and reverse transcription loop-mediated amplification (“RT-LAMP”) for research use only. LAMP is a patented technology belonging to Eiken Chemical Co., Ltd and any other use other than research may require a license from Eiken Chemical Co., Ltd. 

Nucleic acid-based aptamers for use with thermophilic DNA polymerases and reverse transcriptases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the WarmStart® LAMP Kit (DNA & RNA) for Research Use Only (RUO).  Commercial use of this product may require a license from New England Biolabs, Inc. For additional information or to inquire about commercial use, please contact busdev@neb.com.

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