Principle

Reliable purification of viral RNA and DNA from cell-free biological fluids

The NucleoSpin® Virus kit is designed for an efficient isolation of viral RNA and DNA from serum, plasma, or any cell-free biological fluids. RNA and DNA viruses are lysed quickly and efficiently by the chaotropic salt including Lysis Buffer VL. DNA viruses are usually more difficult to lyse and require a digestion with Proteinase K which is supplied in the kit. Carrier RNA improves binding and recovery of low-concentrated viral nucleic acids. Lysis buffer combined with ethanol create an appropriate binding condition for nucleic acids to the NucleoSpin® Virus Columns. The following three washing steps with buffers VW1 and VW2 removes salts, metabolites, and soluble macromolecular cellular components. The nucleic acids are eluted in water and are ready-for-use for subsequent reactions.

Features

• Suitable for liquid cell-free body fluids, such as serum or plasma
• Efficient recovery of viral nucleic acids from 200 µL or up to 400 µL starting volume (two loading steps)
• Nucleic acid isolation from human and animal samples
• Liquid Proteinase K is included – efficient sample lysis

Application Data

High Sensitive BTV viral RNA detection with the NucleoSpin® Virus kit
Viral BTV RNA was isolated from 12 different bovine EDTA-blood samples with different virus titer using NucleoSpin® Virus and a competitor kit Q. BTV RNA was quantified by real-time RT-PCR. The average CT value is lower for NucleoSpin® Virus indicating higher viral RNA yields.

Reliable CMV viral DNA recovery with the NucleoSpin® Virus kit
NucleoSpin® Virus and a competitor kit R were used for viral CMV DNA isolation from different plasma samples with diverse virus titer. The viral DNA were quantified by qPCR in Roche LightCycler 480. The NucleoSpin® Virus shows consistent reliable CMV DNA recovery.

- Kits to be used for research purposes only