Principle

For reliable purification of viral RNA and DNA* from cell-free biological fluids

NucleoSpin® RNA Virus is designed for the isolation of viral nucleic acids from serum, plasma, or any cell-free biological fluids. NucleoSpin® RNA Virus features a special type of membrane which has a high binding capacity for nucleic acids. The procedure is rapid and easy. The lysate is incubated for 5–10 min and applied to the NucleoSpin® column. After two washing steps to remove contaminations the RNA can be eluted with water or low-salt buffer. The kit contains all necessary buffers, spin columns and detailed protocols. Generally, NucleoSpin® RNA Virus is suited for the simultaneous purification of viral RNA and DNA (support protocol). For example, isolation of HBV DNA is performed with an additional Proteinase K digest (enzyme not included in the kit). For isolation of viral DNA with highest sensitivities, the NucleoSpin® Blood kit is recommended.

* For parallel isolation of viral RNA and DNA use of Proteinase K required

Features