NucleoSpin RNA Virus
Product informationBenefits
- Reliable and reproducible isolation of viral RNA and DNA* – free of impurities
- Safe and well-established procedure
- Proven for a large variety of viruses including influenza viruses, HCV, HIV, Bird Flu Virus, Blue Tongue Virus
- Carrier RNA included for highest sensitivity in downstream applications
- Fast and convenient protocol – viral RNA and DNA* within only 30 min
* For parallel isolation of viral RNA and DNA use of Proteinase K required (not included in the kit)
| Code | Name | Size | Quantity | Price | |
|---|---|---|---|---|---|
740956.10 |
NucleoSpin RNA Virus |
10 preps | - | Unavailable in your region | |
740956.50 |
NucleoSpin RNA Virus |
50 preps | - | Unavailable in your region | |
740956.250S |
NucleoSpin RNA Virus Columns |
250 Columns | - | Unavailable in your region | |
740956.240C |
NucleoSpin RNA Virus for QIAcube |
240 preps | - | Unavailable in your region | |
740956.250 |
NucleoSpin RNA Virus |
250 preps | - | Unavailable in your region |
Principle
For reliable purification of viral RNA and DNA* from cell-free biological fluids
NucleoSpin® RNA Virus is designed for the isolation of viral nucleic acids from serum, plasma, or any cell-free biological fluids. NucleoSpin® RNA Virus features a special type of membrane which has a high binding capacity for nucleic acids. The procedure is rapid and easy. The lysate is incubated for 5–10 min and applied to the NucleoSpin® column. After two washing steps to remove contaminations the RNA can be eluted with water or low-salt buffer. The kit contains all necessary buffers, spin columns and detailed protocols. Generally, NucleoSpin® RNA Virus is suited for the simultaneous purification of viral RNA and DNA (support protocol). For example, isolation of HBV DNA is performed with an additional Proteinase K digest (enzyme not included in the kit). For isolation of viral DNA with highest sensitivities, the NucleoSpin® Blood kit is recommended.
* For parallel isolation of viral RNA and DNA use of Proteinase K required
Features
| Technology | Silica-membrane technology |
| Format | Mini spin columns |
| Sample material | 150 µl serum, plasma, cell-free biological fluids |
| Fragment size | 100 bp - approx. 50 kbp |
| Typical recovery | >90% |
| Elution volume | 50 µl |
| Preparation time | 30 min/4-6 preps |
| Binding capacity | 40 µg |
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The supporting documents available for this product can be downloaded below.