Principle

Low profile, 0.1 ml polypropylene wells, polycarbonate frame, cut corner H12, working volume: <100 µl, total well capacity: 200 µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block); extra white wells for improved sensitivity in fluorescent assays.

FrameStar® Roche Style plates are designed to achieve optimised assay conditions on the Roche LightCycler® 96 and 480 (with 96 well block).

This particular style of plate is in a low profile 96-well format; perfect for reaction volumes of 10-100 µl. The wells are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well. This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction. This is especially recommended for reaction volumes below 20 µl.

The extra white wells allow for increased sensitivity in fluorescent assays.

The semi-skirted frame allows for labelling or barcoding.

Barcoding

To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding (see Barcoding for more details).

Please be aware the barcoding products have a minimum order quantity of 10 boxes

Features

• Compatible with the Roche LightCycler® 96 and 480 (with 96 well block)
• Recommended for low volume PCR
• Rigid polycarbonate frame adds to the mechanical stability of the plate - as a result thermal expansion and sample evaporation is reduced significantly, leading to an improved consistency in PCR results
• Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
• Improved seal integrity due to thermal stability of frame
• Compatible with standard multichannel pipettes
• Ideal for use with robotic systems
• <0.1 ml (100 µl) working volume, 0.2 ml (200 µl) total well capacity
• Plates are available with a clear polycarbonate frame and extra white wells for improved sensitivity when using fluorescent based assays
• Raised and colour differentiated grid reference to aid well and sample identification
• Available barcoded
• Combi packs available with qPCR Seal (4ti-0560)

Technical Specifications

_{FrameStar® is covered by one or more of the following US patents or their foreign counterparts, owned by Eppendorf AG: US Patent Nos. 7,347, 977 and 6,340,589. FrameStar® is a registeredtrademark owned by 4titude®Ltd}