Principle

NucleoMag® Pathogen

The NucleoMag® Pathogen kit allows the purification of viral RNA and DNA and microbial DNA using magnetic beads. The kit is designed for common clinical sample material, such as whole blood, serum or plasma, feces, tissue, or swabs. After a sample specific pre-lysis procedure, 200 µL liquid or homogenized sample is used in the purification procedure.

The NucleoMag® product family is based on magnetic bead technology. Nucleic acids are bound to the magnetic beads under chaotropic salt conditions, contaminants are washed away with ethanolic wash buffers, and pure RNA and DNA can be eluted from the beads using elution buffer.

The NucleoMag® technology can easily be adapted to common liquid handling instruments. It can be used either with static pin separators (e.g., NucleoMag® SEP) or magnetic separators integrated into robotic workstations. For more information on adapting any NucleoMag® product to your workstation, please contact your local distributor or MACHEREY-NAGEL directly.

NucleoMag® Pathogen procedure

Features

• One kit for any common clinical sample type
• High sensitivity
• Reliable nucleic acid isolation - suitable even for low viral titers

Applications

• Extraction of viral RNA and DNA and microbial DNA from clinical samples*
• Clinical testing applications*

* Kits to be used for research purposes only