Product Class: Kit

SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit 

For Research Use Only. Not for use in diagnostic procedures.


Catalog #E2019

Product Introduction

The SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit utilizes isothermal amplification for use in the analysis of SARS-CoV-2, the novel coronavirus that causes COVID-19.

  • What is the impact of the Omicron variant on this assay? See the new FAQ and view the Primer Monitor Tool (more info here) for up-to-date information on variants mapped against common primer sets.
  • Colorimetric LAMP enables simple, visual detection (pink-to-yellow) of amplification of SARS-CoV-2 nucleic acid
  • Set up reactions quickly and easily using a simple heat source and unique WarmStart® technology
  • Reduce risk of carryover contamination with thermolabile UDG and dUTP included in the master mix
  • Assay targets N and E regions of the SARS-CoV-2 genome for optimized sensitivity and specificity
  • Bring confidence to your results using the provided controls
  • Components sold separately
  • Learn more about LAMP and other isothermal amplification methods
  • Need assistance designing LAMP primers? Use the NEB LAMP Primer Design Tool
  • Read how NEB developed and implemented a SARS-CoV-2 RT-LAMP workflow for workplace surveillance: 1) NEB Expressions article 2) PLOS ONE publication
  • A publication by members of the global LAMP (gLAMP) Consortium provides a comprehensive review of LAMP and its role in the COVID-19 pandemic
 
 

Protocols, Manuals & Usage

Protocols

  1. SARS-CoV-2 Rapid Colorimetric LAMP Detection Assay Protocol (NEB #E2019)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Application Notes

Tools & Resources

Web Tools

FAQs & Troubleshooting

FAQs

  1. How does the new Omicron variant impact the effectiveness of the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit?
  2. Can the Colorimetric LAMP 2X Master Mix be used in instruments that utilize fluorescent detection of DNA amplification or real time turbidity measurement?
  3. How stable are the WarmStart Colorimetric LAMP 2X Master Mixes?
  4. The WarmStart LAMP Master Mix has some precipitation in the tube after thawing, is this normal?
  5. What if my colorimetric LAMP mix is orange instead of pink prior to amplification?
  6. Does the WarmStart® Colorimetric LAMP Master Mix with UDG enable carryover prevention/contamination reduction?
  7. Does pre-heating the thermal block impact LAMP amplification reactions?
  8. What regions of the SARS-CoV-2 virus do the LAMP Primers target? How were these primers chosen?
  9. In the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit, what is the target of the Internal Control Primer Mix (rActin)?
  10. Should I use Guanidine HCl (B2619A) in colorimetric LAMP reactions?
  11. How are colorimetric LAMP results interpreted in the SARS-CoV-2 Kit?
  12. After amplification my samples turned orange rather than yellow. Is this acceptable? How do I interpret the results?
  13. Can I incubate my colorimetric LAMP Sars-CoV-2 samples longer than 30 minutes at 65°C? How long should I incubate the reaction before checking the results?
  14. My NTC sample(s) turned orange/yellow following incubation at 65°C. What happened?
  15. My colorimetric LAMP SARS-CoV-2 Positive Control failed to turn yellow following incubation at 65°C. What happened?
  16. What is the SARS-CoV-2 Positive Control included in the kit?
  17. How much sample can be added to the colorimetric LAMP reaction as input?
  18. What types of samples/materials are compatible with the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit?
  19. My SARS-CoV-2 sample or Internal Control reaction turned orange/yellow upon sample addition. Does that mean that the sample contains nucleic acid?
  20. What instruments are compatible with the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit?
  21. Can I monitor the color change of the colorimetric LAMP reactions using a spectrophotometer?
  22. Can the WarmStart Colorimetric LAMP 2X Master Mixes be used in instruments that utilize fluorescence detection?

Troubleshooting