Product Class: Other

Deoxynucleotide (dNTP) Solution Mix

Product Introduction

Enhance your PCR Reaction with our ultrapure dNTPs

  • Compatible with all NEB DNA polymerases in a variety of applications
  • ≥ 99% pure as determined by HPLC analysis
  • Exceptional stability
Catalog # Size Concentration
N0447S 8.0 µmol 10 mM
N0447L 40.0 µmol 10 mM

Product Information


Deoxynucleotide (dNTP) Solution Mix is an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP.

A small pack contains 4 vials of 0.2 mls and a large pack contains 5 vials of 0.8 mls.

Supplied in: Ultrapure water as a sodium salt at pH 7.5.

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
This product is related to the following categories:
Nucleotide Solutions Products,
Reverse Transcriptases & RT-PCR Products,
This product can be used in the following applications:
Whole Genome Amplification & Multiple Displacement Amplification,
Strand Displacement Amplification & Nicking Enzyme,
Transcription-Mediated and NASBA Amplification,
Whole Genome Amplification,
DNA Sequencing,
Polymerases for DNA Manipulation,
Hot Start PCR,
Loop-Mediated Isothermal Amplification,
Long Range PCR,
Fast PCR,
RT-PCR & cDNA Synthesis,
Isothermal Amplification,
Multiplex PCR,
Specialty PCR,
Routine PCR,

Properties & Usage

Product Notes

  1. Can be diluted using strerile distilled water, preferably milli-q® water, or sterile TE (10mM Tris-HCl, 1mM EDTA pH 7.5).

Protocols, Manuals & Usage


  1. Loop-mediated Isothermal Amplification (LAMP)


The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at or fill out the Technical Support Form for appropriate document.)

FAQs & Troubleshooting


  1. What is supplied with the Deoxynucleotide Solution Mix?
  2. What is in the Deoxynucleotide Solution Mix?
  3. How stable are the nucleotides?
  4. What is the concentration of Deoxynucleotide Solution Mix?
  5. What polymerases can the Deoxynucleotide Solution Mix be used with?
  6. How does this product differ from the Deoxynucleotide Solutions Set (NEB# N0446)?
  7. If a final concentration of 200 μM each dNTP is called for, how much of the mix should be added to a 100 μl reaction?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.


The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please email us at

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.


NEW ENGLAND BIOLABS® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.

MILLI-Q® is a registered trademark of Millipore Corporation.