Product Class: Other

Bst 3.0 DNA Polymerase
NEBU cloned at NEB recombinant 65 80 Heat

Need assistance designing LAMP primers? Use the NEB LAMP Primer Design Tool.

This is product is available in a glycerol-free format. Contact us for more information.

Product Introduction

Bst 3.0 DNA Polymerase demonstrates robust performance even in high concentrations of amplification inhibitors and features significantly increased reverse transcriptase activity compared to Bst DNA Polymerase.

  • Fused to novel nucleic acid binding domain for enhanced performance
  • Optimized for Loop-Mediated Isothermal DNA Amplification (LAMP)
  • Simplified reaction setup: single-enzyme RT-LAMP reaction
  • High reverse transcriptase activity up to 72°C
  • Offers fast polymerization rates and robust performance even in presence of inhibitors, including dUTP
  • Learn more about LAMP and other isothermal amplification methods
Catalog # Size Concentration
M0374S 1600.0 units 8000 units/ml
M0374L 8000.0 units 8000 units/ml
M0374M 8000.0 units 120000 units/ml

Protocols, Manuals & Usage

Protocols

  1. Typical LAMP Protocol (M0374)

Tools & Resources

Web Tools

FAQs & Troubleshooting

FAQs

  1. What is the difference between Bst DNA Polymerase, Large Fragment, Bst 2.0, and Bst 3.0 DNA Polymerase?
  2. Why would I use Bst 3.0 DNA Polymerase?
  3. Can Bst 3.0 DNA Polymerase be used in other NEBuffers?
  4. How active is Bst 3.0 at other temperatures?
  5. Does Bst 3.0 DNA polymerase incorporate dUTP?
  6. Does Bst 3.0 DNA polymerase have reverse transcriptase activity?
  7. Can Bst 3.0 DNA Polymerase be used to blunt DNA?
  8. Can Bst 3.0 DNA Polymerase be used to fill in 3' overhangs?
  9. Can Bst 3.0 DNA Polymerase be used to remove 5' overhangs?
  10. Can Bst 3.0 DNA Polymerase be heat inactivated?
  11. Are NEB DNA Polymerases supplied with dNTPs?
  12. What are the main causes of reaction failure using Bst 3.0 DNA Polymerase?
  13. Does Bst 3.0 DNA Polymerase have an active 3'→5' proofreading exonuclease?
  14. Can Bst 3.0 DNA Polymerase be used in applications requiring thermal cycling?
  15. Can Bst 3.0 DNA Polymerase initiate at a nick in the DNA?
  16. Can Bst 3.0 DNA Polymerase be used in labeling reactions and partial fill in reactions?
  17. Can Bst 3.0 DNA Polymerase be diluted?
  18. When I thaw Isothermal Amplification Buffer II I see a lot of white precipitate, is this normal?
  19. When should Bst 3.0 DNA Polymerase be the enzyme of choice?
  20. How do I reduce non-template amplification (NTC) in LAMP reactions with Bst 3.0?
  21. How do I use Antarctic Thermolabile UDG for carryover prevention in LAMP reactions?
  22. Does NEB have a master mix for LAMP or RT-LAMP reactions?
  23. What is LAMP and RT-LAMP?