WarmStart® Fluorescent LAMP/RT-LAMP Kit (with UDG)
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
E1708S |
WarmStart Fluorescent LAMP/RT-LAMP Kit with UDG |
100 rxns ( 2 X ) | - | Unavailable in your region | |
E1708L |
WarmStart Fluorescent LAMP/RT-LAMP Kit with UDG |
500 rxns ( 2 X ) | - | Unavailable in your region |
WarmStart® Fluorescent LAMP/RT-LAMP Kit (with UDG)
Need assistance designing LAMP primers? Use the NEB LAMP Primer Design Tool.
Catalog #E1708
Product Introduction
- Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets
- Reduce risk of carryover contamination with thermolabile UDG and dUTP included in the master mix
- Includes fluorescent dye to enable real-time fluorescence measurement of LAMP/RT-LAMP reaction
- WarmStart technology inhibits activity at room temperature
- Learn more about how RT-LAMP is being used for rapid COVID-19 screening
- Master mix (NEB #M1708), LAMP Fluorescent Dye (NEB #B1700) components available separately
- Also available in a lyophilized format for steamlined assay development.
- Product Information
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
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Product Information
Description
The WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG) is designed to provide a simple, one-step solution for real-time detection of LAMP/RT-LAMP reactions via fluorescence. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provide rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase. This kit is supplied with the WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) (NEB #M1708) featuring a blend of Bst 2.0 WarmStart® DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized LAMP buffer solution. Both Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase have been engineered for improved performance in LAMP and RT-LAMP reactions. Also included is a 50X LAMP Fluorescent Dye for real-time fluorescence measurement.
The inclusion of dUTP and thermolabile UDG in the master mix reduces the possibility of carryover contamination, where unintended product of a previous amplification can serve as the substrate of a subsequent reaction. Thermolabile UDG is completely inactivated at temperatures above 50°C, thereby having no effect on the reaction.
This product is also offered in a lyophilized format for ambient/room temperature storage and shipping and streamlined assay development. The lyophilized product has been developed to retain the performance of the E1708 liquid version and is offered in glass vials and a 96-well plate. To learn about the lyophilization process, or to inquire about customizing this product to meet your assay needs, please visit www.neb.com/lyoprime.
- This product is related to the following categories:
- Isothermal Amplification & Strand Displacement Products
- This product can be used in the following applications:
- Loop-Mediated Isothermal Amplification
Kit Components
Kit Components
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration | |||||||||||||||||||
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Properties & Usage
Materials Required but not Supplied
LAMP Primers (we recommend using the NEB LAMP Primer Design Tool)Target nucleic acid samples
Molecular Biology Grade H2O
Heat block, water bath, real-time turbidimeter or thermocycler (with real-time fluorescence measurement if desired) and instrument-appropriate reaction vessels
Related Products
Companion Products
Protocols, Manuals & Usage
Protocols
Manuals
Application Notes
Tools & Resources
Web Tools
FAQs & Troubleshooting
FAQs
- What is the optimal LAMP/RT-LAMP amplification temperature?
- What is the Mg++ concentration in the WarmStart LAMP/RT-LAMP Master Mix?
- How do I use the fluorescent dye?
- What types of input samples/materials are compatible with the LAMP/RT-LAMP mixes?
- How fast should I expect a result?
- How do I confirm a positive result?
- Can I set up my LAMP/RT-LAMP reactions at room temperature?
- The WarmStart LAMP Master Mix has some precipitation in the tube after thawing, is this normal?
- How do I design LAMP Primers?
- What is the difference between NEB #E1708: WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG) and NEB #E1700: WarmStart LAMP Kit (DNA & RNA)?
- What components are included in the WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG)?
- Does the WarmStart LAMP/RT-LAMP 2X Master Mix (with UDG) enable carryover prevention/contamination reduction?
- Amplification occurred in my NTC sample(s) following isothermal incubation. What happened?
- What are the advantages of using standard LAMP (NEB #E1700, M1708, E1708) over pH-based colorimetric LAMP (NEB #M1800, M1804)?
- Is a separate incubation step required for RT-LAMP?
- Does NEB have a master mix for LAMP or RT-LAMP reactions?
- What is LAMP and RT-LAMP?
- What type of purification is recommended for LAMP primers?
Citations & Technical Literature
Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- E1708L_v1_10114221
- E1708S_v1_10114298
- E1708S_v2_10135812
- E1708L_v2_10135818
- E1708L_v2_10137402
- E1708L_v2_10139438
- E1708S_v2_10138396
- E1708S_v2_10141435
- E1708L_v2_10141014
- E1708S_v2_10144803
- E1708S_v2_10145386
- E1708L_v2_10148981
- E1708S_v2_10153100
- E1708L_v2_10155082
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- E1708L_v2_10157245
- E1708S_v2_10158366
- E1708S_v2_10162277
- E1708L_v2_10159701
- E1708S_v2_10164972
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- E1708L_v2_10172530
- E1708L_v2_10180128
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- E1708S_v2_10180127
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- E1708L_v2_10208703
- E1708S_v2_10197178
- E1708S_v2_10208704
- E1708S_v2_10219116
- E1708L_v2_10221458
- E1708S_v2_10232037
- E1708L_v2_10233767
- E1708L_v2_10245013
- E1708S_v2_10242947
- E1708S_v2_10255383
- E1708L_v2_10251189
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.WarmStart® Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG)
LAMP Fluorescent Dye
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Licenses
The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased product to perform loop-mediated amplification (“LAMP”) and reverse transcription loop-mediated amplification (“RT-LAMP”) for research use only. LAMP is a patented technology belonging to Eiken Chemical Co., Ltd and any other use other than research may require a license from Eiken Chemical Co., Ltd.
Nucleic acid-based aptamers for use with thermophilic DNA polymerases and reverse transcriptases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the WarmStart®Fluorescent LAMP/RT-LAMP Kit (with UDG) for Research Use Only (RUO). Commercial use of this product may require a license from New England Biolabs, Inc. For additional information or to inquire about commercial use, please contact busdev@neb.com.
Other Products You May Be Interested In
The supporting documents available for this product can be downloaded below.