Product Class: Other

LAMP Fluorescent Dye

Need assistance designing LAMP primers? Use the NEB LAMP Primer Design Tool


Catalog #B1700

Product Introduction

  • Enables real-time fluorescence measurement of a LAMP reaction
  • Component of the WarmStart® LAMP Kit (DNA & RNA) (NEB #E1700)
  • Sufficient for at least 500 reactions
  • Learn more about LAMP and other isothermal amplification methods
  • Learn more about how RT-LAMP is being used for rapid COVID-19 screening
  • Learn more about how NEB is supporting COVID-19 research
 

Product Information

Description

The LAMP Fluorescent Dye is an intercalating dye that can be used to track a LAMP reaction in real-time by fluorescence measurement. It is provided at 50X concentration and includes an amount sufficient for at least 500 LAMP reactions (0.5 µl per 25 µl LAMP reaction is recommended when monitoring reactions on most real time qPCR instruments). In other instruments, further dilution of the dye may be necessary (to a final concentration of 0.1-0.5X).

The SYBR®/FAM channel of common real-time fluorimeters can be used to read the dye.
This product is related to the following categories:
Isothermal Amplification & Strand Displacement Products
This product can be used in the following applications:
Loop-Mediated Isothermal Amplification,
Isothermal Amplification

Properties & Usage

Protocols, Manuals & Usage

Protocols

  1. WarmStart LAMP Kit (DNA & RNA) Protocol (E1700)

Tools & Resources

Web Tools

FAQs & Troubleshooting

FAQs

  1. How do I use the fluorescent dye?
  2. How can I see the products of a LAMP reaction?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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