WarmStart® RTx Reverse Transcriptase
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
M0380S |
WarmStart RTx Reverse Transcriptase |
50 rxn ( 15000 units/ml ) | - | Unavailable in your region | |
M0380L |
WarmStart RTx Reverse Transcriptase |
250 rxn ( 15000 units/ml ) | - | Unavailable in your region |
WarmStart® RTx Reverse Transcriptase
Need assistance designing LAMP primers? Use the NEB LAMP Primer Design Tool.
This product is available in a glycerol-free format.
Product Introduction
- Ideal for use in Loop-Mediated Isothermal Amplification (LAMP)
- WarmStart® technology enables high-throughput applications, room temperature reaction setup, and increases the consistency and specificity of amplification reactions
- Sensitive RNA detection: 1 pg RNA in LAMP, 10 fg input RNA in RT-PCR
- Optimal reaction temperature from 55-65°C
- One of several products available glycerol-free
Catalog # | Size | Concentration |
---|---|---|
M0380S | 400.0 units | 15000 units/ml |
M0380L | 2000.0 units | 15000 units/ml |
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Product Information
Description
WarmStart RTx Reverse Transcriptase is a unique in silico designed RNA-directed DNA polymerase coupled with a reversibly-bound aptamer that inhibits RTx activity below 40°C. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA (cDNA synthesis) or single-stranded DNA as a template. RTx is a robust enzyme for RNA detection in amplification reactions and is particularly well suited for use in LAMP (loop-mediated isothermal amplification). The WarmStart property enables high throughput applications, room temperature setup, and increases the consistency and specificity of amplification reactions. RTx contains intact RNase H activity.
To support lyophilization, incorporation into microfluidic devices, and enable quick adoption into automation workflows, WarmStart RTx Reverse Transcriptase is also offered in a glycerol-free format.
Total RNA | WarmStart RTx | AMV RT |
100 ng | 3/3 | 3/3 |
10 ng | 3/3 | 3/3 |
1 ng | 3/3 | 3/3 |
100 pg | 3/3 | 2/3 |
10 pg | 3/3 | 2/3 |
1pg | 3/3 | 1/3 |
RT-LAMP reactions were performed with WarmStart RTx and AMV Reverse Transcriptase with total RNA at 6 input concentrations. Positive detection, indicated by fluorescent detection with a twenty-minute reaction time, was achieved down to 1 pg of total input RNA. Use of AMV resulted in false negatives when total RNA was 100 pg or lower.
- This product is related to the following categories:
- RT-PCR Products,
- cDNA Synthesis & Reverse Transcriptases Products,
- This product can be used in the following applications:
- Strand Displacement Amplification & Nicking Enzyme,
- cDNA Synthesis,
- Reverse Transcription (cDNA Synthesis),
- Loop-Mediated Isothermal Amplification,
- RT-PCR & cDNA Synthesis,
- Isothermal Amplification, PCR
Reagents Supplied
Reagents Supplied
The following reagents are supplied with this product:
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Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in a total reaction volume of 50 μl in 20 minutes at 50°C using poly(rA)•oligo(dT)18 as template.Reaction Conditions
1X Isothermal Amplification Buffer Pack
1X Isothermal Amplification Buffer Pack
20 mM Tris-HCl
10 mM (NH4)2SO4
50 mM KCl
2 mM MgSO4
0.1% Tween® 20
(pH 8.8 @ 25°C)
Storage Buffer
100 mM KCl
10 mM Tris-HCl
0.1 mM EDTA
1 mM DTT
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation
80°C for 10 minutesUnit Assay Conditions
20 mM Tris-HCl (pH 8.8), 10 mM (NH4)2SO4, 50 mM KCl, 2 mM MgSO4, 0.1% Tween-20, 0.5 mM dTTP, 0.4 mM poly(rA)•oligo(dT)18.Application Features
- RT-LAMP
- cDNA synthesis
- RT reactions requiring room temperature setup
Related Products
Companion Products
Product Notes
- Reaction Conditions: 1X Isothermal Amplification Buffer, template, primer, dNTPs and 0.25–0.5 µl of WarmStart RTx Reverse Transcriptase in a reaction volume of 25 µl. Incubate at 50–55°C for cDNA synthesis or directly at 65°C for One-step RT-LAMP.
Protocols, Manuals & Usage
Protocols
Tools & Resources
Web Tools
FAQs & Troubleshooting
FAQs
- The product size was previously listed in Reactions and is now listed in Units. What has changed?
- What is LAMP and RT-LAMP?
- What advantages does WarmStart® RTx provide?
- How sensitive is RNA amplification with WarmStart® RTx?
- Can WarmStart® RTx be inactivated?
- Is WarmStart® RTx active in other buffers?
- How do I use WarmStart® RTx in RT-LAMP?
- What is the maximum length of cDNA product produced by WarmStart® RTx?
- How do I use WarmStart® RTx for cDNA synthesis?
- Does WarmStart® RTx have RNase H activity?
- What dNTPs do you recommend for use with WarmStart® RTx?
- Can DNA be used as a template for WarmStart® RTx?
- Does NEB have a master mix for LAMP or RT-LAMP reactions?
Citations & Technical Literature
Citations
Additional Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specification Change Notifications
To standardize product size and provide flexibility to usage, effective April 2, 2024, this product is now sold in terms of Units. This will result in the following changes:
M0380S: 50 Reactions changed to 400 Units
M0380L: 250 Reactions changed to 2,000 Units
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- M0380S_L_v1_0021612
- M0380S_L_v1_0071712
- M0380L_v1_10017040
- M0380L_v2_10018566
- M0380S_v2_10018691
- M0380L_v2_10029517
- M0380L_v2_10043540
- M0380S_v2_10043534
- M0380S_v2_10045185
- M0380L_v2_10049871
- M0380L_v2_10053949
- M0380L_v2_10056288
- M0380L_v2_10061431
- M0380L_v2_10063609
- M0380S_v2_10064062
- M0380L_v3_10068150
- M0380L_v3_10069671
- M0380L_v3_10071274
- M0380L_v3_10072072
- M0380L_v3_10072356
- M0380S_v3_10073333
- M0380L_v3_10072991
- M0380L_v3_10075707
- M0380S_v3_10075708
- M0380L_v3_10077583
- M0380L_v3_10079197
- M0380L_v3_10081060
- M0380L_v3_10081329
- M0380L_v3_10082368
- M0380S_v3_10080031
- M0380L_v3_10084872
- M0380L_v3_10088111
- M0380L_v3_10090042
- M0380L_v3_10093415
- M0380L_v3_10096328
- M0380L_v3_10100859
- M0380L_v3_10104367
- M0380L_v3_10104369
- M0380S_v3_10104074
- M0380L_v3_10110685
- M0380L_v3_10117501
- M0380L_v3_10129536
- M0380L_v3_10131768
- M0380S_v3_10135704
- M0380L_v3_10131911
- M0380L_v3_10145393
- M0380S_v3_10148693
- M0380L_v3_10151378
- M0380L_v3_10155756
- M0380L_v3_10162100
- M0380L_v3_10169325
- M0380S_v3_10169532
- M0380L_v3_10186888
- M0380L_v3_10190677
- M0380L_v3_10205733
- M0380S_v3_10203659
- M0380L_v3_10210682
- M0380L_v3_10213897
- M0380S_L_v1_0021606
- M0380L_v3_10221369
- M0380L_v3_10222864
- M0380S_v3_10226656
- M0380L_v3_10235138
- M0380S_v3_10235139
- M0380S_v3_10244107
- M0380S_v3_10247883
- M0380L_v3_10244108
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.WarmStart® RTx Reverse Transcriptase
Isothermal Amplification Buffer Pack
Magnesium Sulfate (MgSO4) Solution
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Licenses
Nucleic acid-based aptamers for use with reverse transcriptases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the WarmStart® RTx Reverse Transcriptase for Research Use Only (RUO). Commercial use of this product may require a license from New England Biolabs, Inc. For additional information or to inquire about commercial use, please contact busdev@neb.com.Other Products You May Be Interested In
The supporting documents available for this product can be downloaded below.