Tte UvrD Helicase
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
M1202S |
Tte-UvrD Helicase |
50 rxns ( 20 µg/ml ) | - | Unavailable in your region |
Tte UvrD Helicase
Need assistance designing LAMP primers? Use the NEB LAMP Primer Design Tool.
Product Introduction
- Unwinds double-stranded DNA
- Thermostable to 65°C
- Reduces non-specific product formation in isothermal amplification (e.g. LAMP)
Catalog # | Size | Concentration |
---|---|---|
M1202S | 0.5 µg | 20 µg/ml |
- Product Information
- Protocols, Manuals & Usage
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
- Other Products You May Be Interested In
Product Information
Description
Tte UvrD Helicase is a repair helicase capable of unwinding double-stranded DNA, without a requirement for a specific flap or overhang structure, from the thermophilic organism Thermoanaerobacter tengcongensis. It is active on a wide range of DNA substrates and, along with its thermostability (active to 70°C), Tte UvrD Helicase has been demonstrated to be a useful additive for improving specificity of isothermal amplification reactions, particularly in conjunction with the WarmStart® LAMP Kit (DNA & RNA).Product Source
Tte UvrD Helicase is isolated from a strain of E. coli which carries the cloned UvrD gene from Thermoanaerobacter tengcongensis.- This product is related to the following categories:
- PCR, qPCR & Amplification Technologies Products
- This product can be used in the following applications:
- Helicase-dependent Amplification,
- Loop-Mediated Isothermal Amplification,
- DNA Amplification, PCR & qPCR
Reagents Supplied
Reagents Supplied
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration | |||||||||||||||||||||||||
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Properties & Usage
Reaction Conditions
1X Isothermal Amplification Buffer Pack
Supplement with 1 mM ATP
Incubate at 65°C
1X Isothermal Amplification Buffer Pack
20 mM Tris-HCl
10 mM (NH4)2SO4
50 mM KCl
2 mM MgSO4
0.1% Tween® 20
(pH 8.8 @ 25°C)
Usage Concentration
0.2 - 0.4 ng/μlStorage Buffer
10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
0.1% Triton® X-100
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation
80°C for 5 minutesReferences
- An, L., Tang, W., Ranalli, T.A., Kim, H.J., Wytiaz, J., and Kong, H. (2005). Characterization of a thermostable UvrD helicase and its participation in helicase-dependent amplification. J Biol Chem. 280 (32), 28952-8. PubMedID: 15955821
- Mauris, J., and Evans, T.C. Jr. (2010). A human PMS2 homologue from Aquifex aeolicus stimulates an ATP-dependent DNA helicase. J Biol Chem. 285 (15), 11087-92. PubMedID: 20129926
- Vincent, M., Xu, Y., and Kong, H. (2004). Helicase-dependent isothermal DNA amplification. EMBO Rep. 5 (8), 795-800. PubMedID: 15247927
Protocols, Manuals & Usage
Protocols
FAQs & Troubleshooting
FAQs
- Does Tte UvrD Helicase (NEB #M1202) require any specific DNA structure for unwinding?
- How do I use Tte UvrD Helicase for reducing non-template amplification in LAMP reactions
- Is Tte UvrD Helicase compatible with the WarmStart Colorimetric LAMP 2X Master Mix (NEB #M1800)?
- Do I need to add ATP for Tte UvrD Helicase activity?
- Is Tte UvrD Helicase active in other buffers?
- Can I purchase large amounts of an existing Enzyme for Innovation?
- What are Enzymes for Innovation?
Citations & Technical Literature
Citations
Additional Citations
- Zaczek-Moczydłowska, M.A.; Mohamed-Smith, L.; Toldrà, A.; Hooper, C.; Campàs, M.; Furones, M.D.; Bean, T.P.; Campbell, K. (2020) A Single-Tube HNB-Based Loop-Mediated Isothermal Amplification for the Robust Detection of the Ostreid herpesvirus 1. Int J Mol Sci; 21, 6605,
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- M1202_v0_10030797
- M1202S_v1_10035793
- M1202S_v1_10041635
- M1202S_v1_10043679
- M1202S_v1_10048358
- M1202S_v1_10062300
- M1202S_v1_10079151
- M1202S_v1_10088264
- M1202S_v1_10091302
- M1202S_v1_10097606
- M1202S_v1_10115887
- M1202S_v1_10157341
- M1202S_v1_10167937
- M1202S_v1_10221166
- M1202S_v1_10237771
- M1202S_v1_10252568
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.Tte UvrD Helicase
Isothermal Amplification Buffer Pack
Adenosine 5'-Triphosphate (ATP)
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Other Products You May Be Interested In
The supporting documents available for this product can be downloaded below.