Product Class: Other

Tte UvrD Helicase
NEBU cloned at NEB recombinant 65 80 Heat

Need assistance designing LAMP primers? Use the NEB LAMP Primer Design Tool.

Product Introduction



  • Unwinds double-stranded DNA
  • Thermostable to 65°C
  • Reduces non-specific product formation in isothermal amplification (e.g. LAMP)
This is an Enzyme for Innovation (EFI). EFI is a project initiated by NEB to provide unique enzymes to the scientific community in the hopes of enabling the discovery of new and innovative applications. These enzymes have interesting properties and unique specificities.
Catalog # Size Concentration
M1202S 0.5 µg 20 µg/ml

Product Information

Description

Tte UvrD Helicase is a repair helicase capable of unwinding double-stranded DNA, without a requirement for a specific flap or overhang structure, from the thermophilic organism Thermoanaerobacter tengcongensis. It is active on a wide range of DNA substrates and, along with its thermostability (active to 70°C), Tte UvrD Helicase has been demonstrated to be a useful additive for improving specificity of isothermal amplification reactions, particularly in conjunction with the WarmStart® LAMP Kit (DNA & RNA).

Tte UvrD Helicase Eliminates Non-Template Amplification in LAMP Reactions



LAMP reactions were performed using the WarmStart® LAMP Kit (DNA & RNA) (NEB #E1700) and a primer set prone to high levels of non-template amplification. Without Tte UvrD Helicase (left), the positive reaction (+DNA) amplifies in ~8 minutes while the no-template control (NTC) amplifies in ~20 minutes. In the presence of 10 ng Tte UvrD Helicase (right), the positive reaction maintains its rapid amplification time with a slight reduction in total RFU, while the NTC reaction is completely suppressed.


Product Source

Tte UvrD Helicase is isolated from a strain of E. coli which carries the cloned UvrD gene from Thermoanaerobacter tengcongensis.
This product is related to the following categories:
PCR, qPCR & Amplification Technologies Products
This product can be used in the following applications:
Helicase-dependent Amplification,
Loop-Mediated Isothermal Amplification,
DNA Amplification, PCR & qPCR

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Reaction Conditions

1X Isothermal Amplification Buffer Pack
Supplement with 1 mM ATP
Incubate at 65°C

1X Isothermal Amplification Buffer Pack
20 mM Tris-HCl
10 mM (NH4)2SO4
50 mM KCl
2 mM MgSO4
0.1% Tween® 20
(pH 8.8 @ 25°C)

Usage Concentration

0.2 - 0.4 ng/μl

Storage Buffer

10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
0.1% Triton® X-100
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

80°C for 5 minutes

References

  1. An, L., Tang, W., Ranalli, T.A., Kim, H.J., Wytiaz, J., and Kong, H. (2005). Characterization of a thermostable UvrD helicase and its participation in helicase-dependent amplification. J Biol Chem. 280 (32), 28952-8. PubMedID: 15955821
  2. Mauris, J., and Evans, T.C. Jr. (2010). A human PMS2 homologue from Aquifex aeolicus stimulates an ATP-dependent DNA helicase. J Biol Chem. 285 (15), 11087-92. PubMedID: 20129926
  3. Vincent, M., Xu, Y., and Kong, H. (2004). Helicase-dependent isothermal DNA amplification. EMBO Rep. 5 (8), 795-800. PubMedID: 15247927

Protocols, Manuals & Usage

Protocols

  1. Protocol for LAMP Reactions with Tte UvrD Helicase (#M1202)
  2. Protocol for unwinding double stranded DNA with Tte UvrD Helicase (#M1202)

FAQs & Troubleshooting

FAQs

  1. Does Tte UvrD Helicase (NEB #M1202) require any specific DNA structure for unwinding?
  2. How do I use Tte UvrD Helicase for reducing non-template amplification in LAMP reactions
  3. Is Tte UvrD Helicase compatible with the WarmStart Colorimetric LAMP 2X Master Mix (NEB #M1800)?
  4. Do I need to add ATP for Tte UvrD Helicase activity?
  5. Is Tte UvrD Helicase active in other buffers?
  6. Can I purchase large amounts of an existing Enzyme for Innovation?
  7. What are Enzymes for Innovation?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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