LyoPrime WarmStart™ Fluorescent LAMP/RT-LAMP Mix (with UDG)
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
L4401S |
LyoPrime WarmStart Fluorescent LAMP/RT-LAMP Mix (with UDG) |
100 rxns | - | Unavailable in your region | |
L4401P |
LyoPrime WarmStart Fluorescent LAMP/RT-LAMP Mix (with UDG) |
1 x 96 wells | - | Unavailable in your region |
LyoPrime WarmStart™ Fluorescent LAMP/RT-LAMP Mix (with UDG)
View the NEB TV webinar on Lyophilizing Reagents on demand. Please contact us to customize this product.
Catalog #L4401
Product Introduction
- Lyophilized format for ambient/room temperature shipping and storage
- Simple, rapid rehydration (view vial protocol, view 96-well plate protocol)
- Store at room temperature for up to 2 years prior to rehydration
- One-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets
- Reduce risk of carryover contamination with Thermolabile UDG and dUTP included in the master mix
- Includes fluorescent dye to enable real-time fluorescence measurement of LAMP/RT-LAMP reaction
- WarmStart technology inhibits enzyme activity at room temperature
- Product Information
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
- Other Products You May Be Interested In
Product Information
Description
The LyoPrime WarmStart Fluorescent LAMP/RT-LAMP Mix (with UDG) is offered in a lyophilized format, allowing for ambient/room temperature shipping and storage before use. It is a simple, one-step solution for real-time fluorescent detection of LAMP/RT-LAMP reactions. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provide rapid detection of a target nucleic acid using LAMP-specific primers and a strand-displacing DNA polymerase. Featuring a blend of Bst 2.0 WarmStart® DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized buffer solution, this mix offers improved performance. LAMP Fluorescent Dye at 0.5X concentration is included to enable detection of amplification via real time fluorescence. The inclusion of dUTP and thermolabile UDG in the master mix reduces the possibility of carryover contamination, where unintended product of a previous amplification can serve as the substrate of a subsequent reaction. Thermolabile UDG is completely inactivated at temperatures above 50°C, thereby having no effect on the reaction.This product is available in 2 x 50 reaction glass vials or a 96-well plate (1 reaction per well). The plate features twelve partially-attached 8-well strip tubes, allowing for use of the entire plate or partial consumption. The yellow shell frame adaptor supports the use of the plate in a variety of common real-time PCR instruments. Both formats can be customized.
A custom version of this product is available without dye to support alternative detection methods (CRISPR-Cas, end-point visualization such as colorimetric detection via a metal indicator (e.g., hydroxynaphthol blue), etc.) For US customers, please contact your local sales representative for more information. International customers: please contact your local subsidiary or distributor.
- This product is related to the following categories:
- Isothermal Amplification & Strand Displacement Products
- This product can be used in the following applications:
- Isothermal Amplification
Reagents Supplied
Reagents Supplied
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration | |
---|---|---|---|---|---|---|
Properties & Usage
Protocols, Manuals & Usage
Protocols
Tools & Resources
Web Tools
FAQs & Troubleshooting
FAQs
- What is the optimal LAMP/RT-LAMP amplification temperature?
- What is the Mg++ concentration in the WarmStart LAMP/RT-LAMP Master Mix?
- What types of input samples/materials are compatible with the LAMP/RT-LAMP mixes?
- How fast should I expect a result?
- How do I confirm a positive result?
- Can I set up my LAMP/RT-LAMP reactions at room temperature?
- The WarmStart LAMP Master Mix has some precipitation in the tube after thawing, is this normal?
- How do I design LAMP Primers?
- Does the WarmStart LAMP/RT-LAMP 2X Master Mix (with UDG) enable carryover prevention/contamination reduction?
- Amplification occurred in my NTC sample(s) following isothermal incubation. What happened?
- Is a separate incubation step required for RT-LAMP?
- What type of purification is recommended for LAMP primers?
- What is LAMP and RT-LAMP?
- What is the concentration of the LAMP Fluorescent Dye in the LyoPrime WarmStart Fluorescent LAMP/RT-LAMP Mix (with UDG)?
- How should the LyoPrime LAMP mix be stored?
- How do I rehydrate the LyoPrime LAMP mix? Can it be resuspended at a higher concentration?
- When I dissolve the lyophilized product, the solution at first appears turbid. Is this normal?
- For L4401P - Do I need to use the entire LAMP plate in a single experiment? How are the 8-well strip tubes separated?
- For L4401P - How do I store unused 8-well strip tubes after opening the foil pouch? How long can I keep unused 8-well strip tubes?
- For L4401P - How long can I keep the plate or the 8-well strip tubes out of the foil pouch before setting up the reaction?
- For L4401P - What is the purpose of yellow shell adaptor?
- For L4401P - Can I reuse the yellow shell adaptor?
- For L4401P - Why is there a cardboard tray in the package?
Citations & Technical Literature
Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.LyoPrime WarmStart™ Fluorescent LAMP/RT-LAMP Mix (with UDG)
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
The supporting documents available for this product can be downloaded below.