Technology /
separation principle

Magnetic-bead technology /
chaotropic salt binding

Material

Superparamagnetic beads (non-silica)

Format

Flexible

Procedure

Principle: binding (high-salt) – washing – elution (low-salt)

• Adsorption of DNA / RNA in the presence of chaotropic salts
(hydrate shell of DNA / RNA is reversibly removed)
• High-salt / ethanolic washing steps to remove contaminants
• Low-salt or water elution (hydrate shell is recovered, DNA is released
from the beads)

Features/Result

Highly-pure ready-to-use PCR products, genomic DNA, RNA
Easily adapted to automated use, e.g. on KingFisher®, KingFisher® mL,
KingFisher® 96, and KingFisher® Flex