Principle

The NucleoMag®  DNA Food

The NucleoMag®  DNA Food kit is designed for the isolation of DNA from food and feed samples, preferably of plant or animal origin. The procedure is based on reversible adsorption of nucleic acids to paramagnetic beads under appropriate buffer conditions. Sample lysis is achieved by incubation of samples with Proteinase K at 65 °C. Lysis mixtures should be cleared by centrifugation or filtration in order to remove contaminants and residual cellular debris. For binding of nucleic acids to the paramagnetic beads, Binding Buffer CB and the NucleoMag® B-Beads are added to the clear lysate. After magnetic separation, the paramagnetic beads are washed to remove contaminants and salts using Wash Buffers CMW, CQW, and 80 % ethanol. Residual ethanol from previous wash steps is removed by air drying. Next, highly purified DNA is eluted with low-salt Elution Buffer (CE) and can be used directly for downstream applications. The NucleoMag®  DNA Food kit can be used either manually, or automated on standard liquid handling instruments and automated magnetic separators.

NucleoMag® DNA Food procedure

Features

• DNA extraction from food and feed for e.g., species identification, GMO detection
• Get even low amounts of partially degraded DNA from complex matrices
• Extraction of DNA from contaminating bacteria (food safety)
• Kit chemistry allows full sample flexibility
  

Applications

• Manual or automated isolation of DNA from food and feed
• For identification of GMO-DNA or animal components
• Typical downstream application: real-time PCR

* Kits to be used for research purposes only