Magnetic-bead based isolation of viral RNA / DNA and bacterial DNA

The NucleoMag VET kit is a magnetic bead-based kit for the manual or automated extraction of viral RNA/DNA and bacterial DNA from veterinary sample specimens such as serum, plasma, cell-free biological fluids, animal whole blood, tissue, swabs, milk and feces. After sample treatment, samples can be processed with the standard purification protocol.

• Inhibitor free viral RNA/DNA and bacterial DNA extraction
• Highest flexibility for various veterinary sample types
• Same standard protocol for all specimen matrices after sample pretreatment
• Easy to automate on magnetic rod or liquid handler systems

Reliable detection of BTV, CSFV, PCV 2, and SBV after DNA or RNA isolation using the NucleoMag VET kit

The NucleoMag VET kit was used to extract viral nucleic acids from bovine blood (A), porcine blood (B), porcine tissue (c) or bovine serum (d). Subsequently, qPCR-analysis was performed to determine the viral titer load in the sample materials: (A) BTV (Bluetongue virus); (B) CSFV (Classical Swine Fever virus) (C) PCV 2 (Porcine Circovirus Type 2); (D) SBV (Schmallenberg virus). Extraction performance was compared to other commercial nucleic acid extractions kits available for isolation of viral RNA and/or DNA. The qRT-PCR data show that DNA or RNA concentration varies on the extraction kit used, which results in a dissimilar detection of BTV, CSFV, PCV 2, and SBV. For the detection of the various viruses in the disparate sample materials, it is shown that DNA or RNA concentration was always high using the NucleoMag VET compared to the other kits. The obtained Ct values for the viral nucleic acids and sample materials show that the NucleoMag VET kit is a very consistent and reliable extraction kit, obtaining low Ct values for a reliable virus detection independent from virus type or sample material.
Source: Application note NucleoMag VET on KingFisher; data was kindly provided by Dr. Hoffmann, Friedrich-Loeffler-Institut, Germany.

 

Cited in over 100 peer-reviewed publications!

The NucleoMag VET kit has been cited in more than 100 peer-reviewed publications. The magnetic bead-based kit has been used in research and molecular diagnostics for a variety of pathogens relevant to veterinary medicine. Click here to see the full reference list

• African/classical swine fever virus
• Lumpy skin disease virus
• Petits ruminants virus
• Influenza A virus
• Foot-and-mouth disease virus
• Schmallenberg virus

 

Features

Technology	Magnetic-bead technology
Format	Highly reactive superparamagnetic beads
Processing	Manual or automated
Sample material	< 200 µL whole blood, serum, plasma, < 10–30 mg tissue (e.g., ear notches), < 200 µL feces, < 200 µL swab wash solution
Maximum amount of starting material in purification procedure	200 µL liquid/homogenized sample
Fragment size	300 bp–approx. 50 kbp
Elution volume	50 - 100 µl
Preparation time	45–120 min/96 preps (45 min for KingFisher®)
Binding capacity	0.4 μg/μL beads

 

 

Learn more

For more detailed information and documents, click here.