Features

Magnetic-bead based isolation of genomic DNA from FFPE samples
• Blue colored Paraffin Dissolver (patent pending) for convenient paraffin removal
• No need for organic solvents
• Removal of formalin crosslinking by efficient decrosslinking buffer
• High quality DNA for improved performance in downstream applications (e.g., PCR)
• Magnetic-bead technology allows highest flexibility, easy adaption for automated use

Applications*

• Rapid isolation of DNA from formalin-fixed, paraffin-embedded samples
• Isolation of DNA from fresh and archived FFPE samples
• Isolation of DNA from specimen of object slides
• Typical downstream application: PCR
* Kits to be used for research purposes only!

Principle / Procedure

NucleoMag® DNA FFPE kit is designed for high-throughput DNA purification from formalin-fixed, paraffin-embedded samples. 
The NucleoMag® DNA FFPE kit contains the odorless Paraffin Dissolver (patent pending) which allows effective lysis in a convenient two-phase system without use of organic solvents. 
First, after dissolving of paraffin from FFPE sections with Paraffin Dissolver, the tissue sample is digested by Proteinase K to solubilize the fixed tissue and release DNA into solution. Subsequently, heat incubation with a specially designed buffer effectively eliminates crosslinks from the previously released DNA.
The procedure is based on the reversible adsorption of nucleic acids to paramagnetic beads under appropriate buffer conditions. After magnetic separation, the paramagnetic beads are washed to remove contaminants and salts using two different wash buffers. Pure genomic DNA is finally eluted under low ionic strength conditions in a slightly alkaline elution buffer.

NucleoMag® DNA FFPE procedure

Consistent DNA yield
Genomic DNA was purified in triplicates from embedded mouse lung, liver, and kidney tissue sections with NucleoMag® DNA FFPE and two competitor kits. The NucleoMag® DNA FFPE kit achieves higher DNA yields compared to competitor kits (A, I).

Excellent PCR performance
Purified genomic DNA was analyzed with LightCycler® PCR and DyNAmoTM Capillary SYBR® Green qPCR Kit (amplicon size 191 bp). Competitor A (A) and competitor I (B) show higher CT values indicating a lower DNA yield compared to samples purified with NucleoMag® DNA FFPE.