Magnetic-bead based isolation of RNA from tissue and cell samples

Principle

NucleoMag® 96 RNA is designed for rapid manual and automated small-scale preparation of highly pure total RNA from tissue or cell samples. Typically, 96 samples can be purified in less than 120 minutes on common automation platforms. The kit provides reagents for the purification of up to 30 µg pure RNA from suitable samples (e.g., 20 mg tissue or 2x106 cells). Depending on the elution volume used concentrations of 10–30 ng/µL can be obtained. Recombinant DNase is included in the kit to obtain RNA of highest purity.

The NucleoMag® product family is based on magnetic bead technology. The binding technology is similar to the silica membrane technology: DNA is bound to the magnetic beads under chaotropic salt conditions, contaminants are washed away with ethanolic wash buffers, and pure RNA can be eluted from the beads using elution buffer.

The NucleoMag® technology can be easily adapted to common liquid handling instruments. It can be used either with static-pin separators (e.g., NucleoMag® SEP) or magnetic separators integrated into robotic workstations. For more information on adapting any NucleoMag® product to your work-station please contact your local distributor or MACHEREY-NAGEL directly.

Features