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NucleoMag® Pathogen

Product information
Code Name Size Quantity Price
744210.1
NucleoMag® Pathogen
1 x 96 preps    - Unavailable in your region
744210.4
NucleoMag® Pathogen
4 x 96 preps    - Unavailable in your region

Principle

NucleoMag® Pathogen

The NucleoMag® Pathogen kit allows the purification of viral RNA and DNA and microbial DNA using magnetic beads. The kit is designed for common clinical sample material, such as whole blood, serum or plasma, feces, tissue, or swabs. After a sample specific pre-lysis procedure, 200 µL liquid or homogenized sample is used in the purification procedure.

The NucleoMag® product family is based on magnetic bead technology. Nucleic acids are bound to the magnetic beads under chaotropic salt conditions, contaminants are washed away with ethanolic wash buffers, and pure RNA and DNA can be eluted from the beads using elution buffer.

The NucleoMag® technology can easily be adapted to common liquid handling instruments. It can be used either with static pin separators (e.g., NucleoMag® SEP) or magnetic separators integrated into robotic workstations. For more information on adapting any NucleoMag® product to your workstation, please contact your local distributor or MACHEREY-NAGEL directly.

NucleoMag® Pathogen procedure

NucleoMag® Pathogen procedure

Features

  • One kit for any common clinical sample type
  • High sensitivity
  • Reliable nucleic acid isolation - suitable even for low viral titers

Technology

Magnetic-bead technology

Format

Highly reactive superparamagnetic beads

Processing

Manual or automated

Sample material

The prep is fully scalable.
A convenient volume, especially for 96-well processing would be:
< 200 µL whole blood, serum, plasma,
< 25 mg tissue (e.g., ear notches),
< 200 µL feces, < 200 µL swab wash solution

Maximum amount of starting material in purification procedure

200 µL liquid/homogenized sample

Fragment size

300 bp–approx. 50 kbp

Elution volume

50–100 µL

Preparation time

45–120 min/96 preps (45 min for KingFisher® Flex)

Binding capacity

0.4 μg/μL beads

 

Applications

• Extraction of viral RNA and DNA and microbial DNA from clinical samples*
• Clinical testing applications*

* Kits to be used for research purposes only



For more detailed information and documents, click here.

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