Product Class: Other

Monarch® gDNA Nuclei Prep & Lysis Buffer Pack


Catalog #T3054

Product Introduction

  • Contains Monarch gDNA Nuclei Prep Buffer (NEB #T3052) and Monarch gDNA Nuclei Lysis Buffer (NEB #T3053), both supplied as components of the Monarch HMW DNA Extraction Kit for Cells & Blood (NEB #T3050)
  • Used for rapid and efficient lysis of mammalian cells 
  • 2-step lysis enables highly effective RNA removal
  • 20 ml of each buffer is included in the buffer pack
  • Monarch gDNA Nuclei Prep Buffer is used pre-chilled to 4°C and can be stored at that temperature for convenience if desired

Product Information

Description

The Monarch gDNA Nuclei Prep & Lysis Buffer Pack contains two lysis buffers used in the Monarch HMW DNA Extraction Kit for Cells & Blood (NEB #T3050). The Nuclei Prep Buffer is a mild lysis buffer used first to lyse the cell membrane and release the contents of the cytoplasm while the nuclei are kept intact. It is used pre-mixed with the RNase A enzyme included in the kit. After the RNA from the cytoplasm is digested by RNase A, the Nuclei Lysis Buffer, pre-mixed with the included Proteinase K, is used to lyse the nuclei and release the HMW genomic DNA. This two-step lysis ensures that RNA in the cytoplasm is effectively degraded before the viscous genomic DNA from the nuclei is released. 20 ml of each buffer is included in this buffer pack. The Nuclei Prep Buffer is used chilled to 4°C, and it can be stored at 4°C for convenience if desired.
This product is related to the following categories:
Genomic DNA Extraction & Purification Products,
Nucleic Acid Purification Products,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • T3054L     25    
      Monarch® gDNA Nuclei Lysis Buffer T3053-1 25 2 x 10 ml Not Applicable
      Monarch® gDNA Nuclei Prep Buffer T3052-1 25 2 x 10 ml Not Applicable

Properties & Usage

Protocols, Manuals & Usage

Protocols

  1. Download Quick Protocol Card for Cells
  2. Download Quick Protocol Card for Blood
  3. Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Cells (NEB #T3050)
  4. Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Blood (NEB #T3050)
  5. Protocol for UHMW DNA Cleanup in the Oxford Nanopore Technologies® UL Library Prep Workflow

FAQs & Troubleshooting

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information about commercial rights, please email us at gbd@neb.com While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

Other Products You May Be Interested In