Product Class: Kit

Monarch® RBC Lysis Buffer


Catalog #T3051

Product Introduction

  • Supplied as a component of the Monarch HMW DNA Extraction Kit for Cells & Blood (NEB #T3050)
  • Specifically lyses erythrocytes (red blood cells) of blood samples, leaving leukocytes intact
  • Removes nucleases and allows hemoglobin to be removed prior to leukocyte lysis
  • Separate RBC lysis enables purification of highly-pure DNA > 1 Mb 
  • RBC Lysis also enables blood inputs up to 2 ml
  • Store and use at 4°C

 

 

Product Information

Description

The Monarch RBC Lysis Buffer is specifically designed to lyse red blood cells (erythrocytes) while leaving leukocytes intact. The separate RBC lysis step enables removal of hemoglobin to increase the purity of the blood prep. The RBC Lysis Buffer also removes nucleases to enable the subsequent purification of HMW DNA into the megabase (Mb) range in size.
This product is related to the following categories:
Genomic DNA Extraction & Purification Products,
Nucleic Acid Purification Products,

Properties & Usage

Protocols, Manuals & Usage

Protocols

  1. Download Quick Protocol Card for Blood
  2. Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Blood (NEB #T3050)
  3. Protocol for UHMW DNA Cleanup in the Oxford Nanopore Technologies® UL Library Prep Workflow
  4. Protocol for Isolation of Genomic DNA (gDNA) from up to 500 µl mammalian blood using the Monarch® Spin gDNA Extraction Kit (NEB #T3010) and RBC Lysis Buffer (NEB #T3051)

Application Notes

FAQs & Troubleshooting

FAQs

  1. Will extended binding increase the yield of the target protein in the eluate?
  2. Can the Monarch HMW DNA Extraction Kits be used to process human Buffy coat?
  3. My blood sample is frozen in aliquots larger than is recommended in your protocol (e.g., in Vacutainers). Can I still use the frozen blood protocol for the Monarch HMW DNA Extraction Kit for Cells & Blood (NEB #T3050)?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information about commercial rights, please email us at gbd@neb.com While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

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