Product Class: Other

MidRange PFG Marker

Product Introduction

  • Suitable for pulsed field gel electrophoresis
  • Size range: 15–291 kb
  • Embedded in 1% Low Melting Point (LMP) agarose
  • Supplied in a GelSyringe™ dispenser
  • Suitable for 50 gel lanes
Catalog # Size Concentration
N0342S 50.0 gel lanes (0.05 ml) 50 µg/ml

Product Information

Description

MidRange PFG Marker consists of concatemers of λ DNA isolated from the bacteriophage λ (cI857 ind1 Sam7) mixed with XhoI digested λ DNA embedded in 1% LMP agarose and supplied in a GelSyringe™ dispenser. XhoI produces fragments of 15.0 and 33.5 kb. These fragments anneal to and form concatemers with intact λ DNA. It is designed for use as a size marker for pulsed field gel electrophoresis (PFG). Size range: 15-291 kb.

N3551_thumb
This product is related to the following categories:
DNA Markers & Ladders Products
This product can be used in the following applications:
DNA Analysis

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Bases

Fragment bp
1 15000
2 33500
3 48500
4 63500
5 82000
6 97000
7 112000
8 130500
9 145500
10 160500
11 179000
12 194000
13 209000
14 227500
15 242500
16 257500
17 276000
18 291000

Effective Size Range

15,000bp to 291,000bp

Storage Buffer

10 mM Tris-HCl
1 mM EDTA
50% Glycerol
1% LMP agarose
pH 8 @ 25°C

Product Notes

  1. The photograph represents the pulsed field gel separation of Mid Range PFG Marker using a CHEF apparatus. The 1% agarose gel was run at 6 volts/cm using ramped pulse times from 1 to 25 seconds for 24 hours at 15°C in 0.5X TBE (50 mM Tris-HCl, 50 mM boric acid, 1 mM EDTA) made with Milli-Q™ water.
  2. Extrude agarose from GelSyringe carefully and slice plugs from the end with a sharp blade. One plug is sufficient for one lane of a gel. Place the plug at the front of the well and seal with molten agarose just above gelling temperature (~42–45°C). Allow no bubbles to form.
  3. Recommended plug sizes are from 5-10 µl. A 10 µl plug (one small graduation on the GelSyringe volume scale) contains approximately 0.5µg of DNA. Each GelSyringe yields 50+ plugs.
  4. Melting plugs will cause denaturation of concatemers.
  5. Never attach the agarose plugs to the gel comb before the gel is poured. Heat from the solidifying gel will cause the Lambda concatemers to denature.

References

  1. Ellard, J., Greci, J. and Davis, T.B. unpublished observation.

FAQs & Troubleshooting

FAQs

  1. Why am I seeing poor results when running the PFG marker on my pulse field gel?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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