Product Class: Other

Monarch® DNA Capture Beads

Product Introduction

  • Specialized 4 mm borosilicate glass beads 
  • Smooth surface enables easy and efficient elution, and HMW DNA is ready to use soon after extraction
  • Optimized for the purification of high molecular weight (HMW) DNA in the Monarch HMW DNA extraction workflows
  • Use with the Monarch Bead Retainers (NEB #T3004) and Monarch Collection Tubes II (NEB #T2018) for best results



Check out our publication in Biotechniques outlining a simple approach to accurate quantitation of ultra high molecular weight (UHMW) DNA.

Catalog # Size Concentration
T3005L 200.0 beads

Product Information

Description

Monarch DNA Capture beads are provided as a component of the Monarch HMW DNA Extraction Kits (NEB #T3050 and #T3060). The specialized beads are 4 mm in diameter and made from borosilicate glass and have been optimized for capturing high molecular weight DNA in the Monarch HMW DNA Extraction workflows. The use of large glass beads in the HMW DNA extraction workflow enables fast and efficient washing with no drying steps required. Elution from the beads is fast and complete; all or nearly all of the DNA is recovered. DNA eluted from the beads is also easy to dissolve and ready to use in significantly less time than other HMW DNA extraction methods. The beads should be used in conjunction with the Monarch Bead Retainers (NEB #T3004) and Monarch Collection Tubes II (NEB #T2018) for convenient handling and for best results.

The Monarch HMW DNA Extraction Kits enable complete recovery of input DNA with the use of smooth glass beads.



NEB #T3050: 10 μg lambda DNA (~20 μl, NEB #N3011) was combined with Monarch gDNA Nuclei Prep Buffer to bring the total volume to 50 μl. The Cell Protocol (low input) was carried out from Step 4, omitting enzymes and enzyme incubation steps. NEB #T3060: 10 μg lambda DNA (~20 μl, NEB #N3011) was combined with Monarch HMW gDNA Tissue Lysis Buffer to a total volume of 100 μl. The Tissue Protocol (for ‘very low input’ samples) was carried out from Step 8, while omitting enzymes and enzyme incubation steps. DNA concentration of 100 μl eluates from each kit was measured by OD assessment. Average recovery rate and standard deviation were calculated based on 8-fold replicates.

 
This product is related to the following categories:
Genomic DNA Extraction & Purification Products,
Nucleic Acid Purification Products,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Protocols, Manuals & Usage

Protocols

  1. Download Quick Protocol Card for Cells
  2. Download Quick Protocol Card for Blood
  3. Download Quick Protocol Card for Tissue
  4. Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Cells (NEB #T3050)
  5. Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Blood (NEB #T3050)
  6. Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Tissue (NEB #T3060)
  7. Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Bacteria (NEB #T3060)
  8. Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Yeast (NEB #T3060)
  9. Protocol for UHMW DNA Cleanup in the Oxford Nanopore Technologies® UL Library Prep Workflow

Usage & Guidelines

Application Notes

FAQs & Troubleshooting

FAQs

  1. I dropped a few of my glass beads. Can I clean them, and do you supply extra?
  2. Is there anything special about the Monarch DNA Capture Beads, or can I use any glass beads?
  3. How do I know that my HMW DNA has attached to glass beads in the Monarch HMW DNA Extraction Kits?
  4. Is there any benefit to adding in a size selection (e.g., Circulomics® Short Read Eliminator) following extraction?
  5. Can the Monarch HMW DNA Extraction Kit be used for processing yeast and/or fungal samples?
  6. Can the Monarch HMW DNA Extraction Kits be used to process human Buffy coat?
  7. Can the Monarch HMW DNA Extraction Kits be used to process environmental or fecal samples for metagenomic studies?
  8. Can the Monarch HMW DNA Extraction Kits be used to isolate BAC DNA?
  9. Does the Monarch HMW DNA Extraction workflow work for single cells?
  10. Can the Monarch HMW DNA Extraction Kits be used to process nematodes?
  11. I am having trouble dissolving my HMW DNA after isolation. Do you have any tips on increasing the solubility?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information about commercial rights, please email us at gbd@neb.com While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

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