Monarch® DNA Capture Beads
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
T3005L |
Monarch DNA Capture Beads |
200 pieces | - | Unavailable in your region |
Monarch® DNA Capture Beads
Catalog #T3005
Product Introduction
- Specialized 4 mm borosilicate glass beads
- Smooth surface enables easy and efficient elution, and HMW DNA is ready to use soon after extraction
- Optimized for the purification of high molecular weight (HMW) DNA in the Monarch HMW DNA extraction workflows
- Use with the Monarch Bead Retainers (NEB #T3004) and Monarch Collection Tubes II (NEB #T2018) for best results
Check out our publication in Biotechniques outlining a simple approach to accurate quantitation of ultra high molecular weight (UHMW) DNA.
- Product Information
- Protocols, Manuals & Usage
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
- Other Products You May Be Interested In
Product Information
Description
Monarch DNA Capture beads are provided as a component of the Monarch HMW DNA Extraction Kits (NEB #T3050 and #T3060). The specialized beads are 4 mm in diameter and made from borosilicate glass and have been optimized for capturing high molecular weight DNA in the Monarch HMW DNA Extraction workflows. The use of large glass beads in the HMW DNA extraction workflow enables fast and efficient washing with no drying steps required. Elution from the beads is fast and complete; all or nearly all of the DNA is recovered. DNA eluted from the beads is also easy to dissolve and ready to use in significantly less time than other HMW DNA extraction methods. The beads should be used in conjunction with the Monarch Bead Retainers (NEB #T3004) and Monarch Collection Tubes II (NEB #T2018) for convenient handling and for best results.- This product is related to the following categories:
- Genomic DNA Extraction & Purification Products,
- Nucleic Acid Purification Products,
Properties & Usage
Related Products
Companion Products
- Monarch HMW DNA Extraction Kit for Cells and Blood
- Monarch HMW DNA Extraction Kit for Tissue
- Monarch Pestle Set
- Monarch gDNA Nuclei Prep and Lysis Buffer Pack
- Monarch RBC Lysis Buffer
- Monarch gDNA Elution Buffer II
- Monarch HMW gDNA Tissue Lysis Buffer
- Monarch Protein Separation Solution
- Monarch 2 ml Tubes
- Monarch Bead Retainers
- Monarch Precipitation Enhancer
- Monarch gDNA Wash Buffer
Protocols, Manuals & Usage
Protocols
- Download Quick Protocol Card for Cells
- Download Quick Protocol Card for Blood
- Download Quick Protocol Card for Tissue
- Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Cells (NEB #T3050)
- Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Blood (NEB #T3050)
- Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Tissue (NEB #T3060)
- Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Bacteria (NEB #T3060)
- Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Yeast (NEB #T3060)
- Protocol for UHMW DNA Cleanup in the Oxford Nanopore Technologies® UL Library Prep Workflow
Usage & Guidelines
- Choosing an Agitation Speed During Lysis with the Monarch HMW DNA Extraction Kits
- Choosing Input Amounts for the Monarch HMW DNA Extraction Kits
- Considerations and Performance Data for Nanopore Sequencing of High Molecular Weight DNA (HMW DNA)
- Important Considerations for Starting Materials When Using the Monarch HMW DNA Extraction Kit for Cells & Blood (NEB #T3050)
- Important Considerations for Starting Materials When Using the Monarch HMW DNA Extraction Kit for Tissues (NEB #T3060)
Application Notes
FAQs & Troubleshooting
FAQs
- I dropped a few of my glass beads. Can I clean them, and do you supply extra?
- Is there anything special about the Monarch DNA Capture Beads, or can I use any glass beads?
- How do I know that my HMW DNA has attached to glass beads in the Monarch HMW DNA Extraction Kits?
- Is there any benefit to adding in a size selection (e.g., Circulomics® Short Read Eliminator) following extraction?
- Can the Monarch HMW DNA Extraction Kit be used for processing yeast and/or fungal samples?
- Can the Monarch HMW DNA Extraction Kits be used to process human Buffy coat?
- Can the Monarch HMW DNA Extraction Kits be used to process environmental or fecal samples for metagenomic studies?
- Can the Monarch HMW DNA Extraction Kits be used to isolate BAC DNA?
- Does the Monarch HMW DNA Extraction workflow work for single cells?
- Can the Monarch HMW DNA Extraction Kits be used to process nematodes?
- I am having trouble dissolving my HMW DNA after isolation. Do you have any tips on increasing the solubility?
Troubleshooting
Citations & Technical Literature
Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Licenses
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information about commercial rights, please email us at gbd@neb.com While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
The supporting documents available for this product can be downloaded below.