Product Class: Other

Monarch® DNA/RNA Protection Reagent

Product Introduction

  • Preserves and stabilizes RNA and DNA
  • A component of the Monarch® Total RNA Miniprep Kit (NEB #T2010)
  • Supplied as a 2X concentrate
  • Supplied in 2 bottles, each containing 28 ml
Catalog # Size Concentration
T2011L 56.0 ml

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Product Information

Description


Monarch® DNA/RNA Protection Reagent is a component of the Monarch Total RNA Miniprep Kit. It is a solution for the preservation of nucleic acids in various tissue types. It is supplied as a 2X concentrate. This reagent does not need to be removed before RNA extraction with the Monarch Total RNA Miniprep Kit.

Figure 1: Monarch DNA/RNA Protection Reagent preserves RNA integrity for up to 1 month at 4°C


Samples were incubated in Monarch DNA/RNA Protection Reagent (NEB #T2011) at 4°C for the indicated time and subsequently processed using the Monarch Total RNA Miniprep Kit (NEB #T2010). Samples were eluted in 100 µl nuclease-free water. Rat liver and blood samples were diluted 5-fold (HeLa cells were not diluted), and 1 µl of each was loaded onto a Bioanalyzer Nano chip (HeLa and rat liver) or Pico chip (blood). The RIN values demonstrate the ability of the Protection Reagent to preserve RNA integrity over time.

This product is related to the following categories:
Total RNA Extraction & Purification Products,
RNA Extraction and Purification,
Nucleic Acid Purification Products,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Protocols, Manuals & Usage

Protocols

  1. Quick Protocol for Total RNA Extraction (NEB #T2010)
  2. Total RNA Purification from Samples Stored in Monarch DNA/RNA Protection Reagent using the Monarch Total RNA Miniprep Kit (NEB #T2010)
  3. RNA Extraction from Buccal/Nasopharyngeal Swabs Using the Monarch RNA Cleanup Kits
  4. RNA Extraction from Saliva Using the Monarch RNA Cleanup Kits
  5. Automation of Viral RNA Extraction from Saliva Using the Monarch Spin RNA Cleanup Kit
  6. Automation of RNA Extraction from Saliva Using the Monarch Total RNA Miniprep Kit
  7. Protocol for Genomic DNA Extraction from Cultured Cells Stabilized with Monarch® DNA/RNA Protection Reagent (NEB #2011) using the Monarch Spin gDNA Extraction Kit (NEB #T3010)
  8. Protocol for Genomic DNA Extraction from Blood Stabilized with Monarch® DNA/RNA Protection Reagent (NEB #T2011) using the Monarch Spin gDNA Extraction Kit (NEB #T3010)
  9. Protocol for Genomic DNA Extraction from Tissue Samples Stabilized with Monarch® DNA/RNA Protection Reagent (NEB #T2011) using the Monarch Spin gDNA Extraction Kit (NEB #T3010)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Usage & Guidelines

Application Notes

FAQs & Troubleshooting

FAQs

  1. How can I preserve my sample if I am not ready to extract RNA right after harvest?
  2. Can the Monarch Total RNA Miniprep Kit (NEB #T2010) be used to purify RNA from saliva?
  3. Can the Monarch Total RNA Miniprep Kit NEB T2010 be used to purify RNA from buccal (cheek) swabs?
  4. Can the Monarch Total RNA Miniprep Kit (NEB #T2010) be used to purify RNA from plasma or serum?
  5. Can the Monarch Total RNA Miniprep Kit (NEB #T2010) be used to purify RNA from nucleated blood (e.g. bird, fish, reptile)?
  6. Can the Monarch Total RNA Miniprep Kit be used to purify RNA from zebrafish embryos/larvae?
  7. Can I lyse my adherent cells directly in their wells when using the Monarch Total RNA Miniprep Kit?
  8. Can extraction of RNA with the Monarch kits be automated?
  9. Is live SARS-CoV-2 virus inactivated during extraction with the Monarch Total RNA Miniprep Kit?
  10. Are the Monarch HMW DNA Extraction kits compatible with samples stored in Monarch DNA/RNA Protection Reagent, RNAlater, DNA/RNA Shield etc.? 

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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