Product Class: Other

NEBNext® Poly(A) mRNA Magnetic Isolation Module

An Express protocol option is now available in the product manual, providing substantial time savings with a more streamlined workflow.

Product Introduction

Ribosomal RNAs (rRNAs) are extremely abundant, constituting 80–90% of total RNA, and efficient removal of rRNA is critical to enable cost-effective sequencing of RNA samples. Oligo dT-based mRNA isolation is ideal for enrichment of mRNA and separation from rRNA when samples are eukaryotic and have intact poly(A) tails.

This product is compatible with total RNA inputs of 1-5µg. For input amounts >5µg we recommend the NEBNext High Input Poly(A) mRNA Isolation Module (NEB #E3370).

Catalog # Size Concentration
E7490S 24.0 reactions
E7490L 96.0 reactions

Product Information

Description

The NEBNext Poly(A) mRNA Magnetic Isolation Module is designed to isolate intact poly(A)+ RNA from 1-5µg of previously isolated total RNA. The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for automated high-throughput applications. Additionally, magnetic separation technology permits elution of intact mRNA in small volumes eliminating the need for precipitating the poly(A)+ transcripts in the eluent. Intact poly(A)+ RNA which is fully representative of the mRNA population of the original sample can be obtained in less than one hour.

Note that for input amounts >5µg we recommend the NEBNext High Input Poly(A) mRNA Isolation Module (NEB #E3370).


This product is related to the following categories:
RNA Depletion & mRNA Enrichment Products,
RNA Library Prep for Illumina,
Next Generation Sequencing Library Preparation Products,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • E7490S     4    
      NEBNext RNA Binding Buffer (2X) E7492AVIAL 4 1 x 7.2 ml Not Applicable
      NEBNext Wash Buffer E7493AVIAL 4 1 x 28.8 ml Not Applicable
      Nuclease-free Water E7495AVIAL 4 1 x 1.2 ml Not Applicable
      NEBNext Tris Buffer E7496AVIAL 4 1 x 6 ml Not Applicable
      NEBNext Oligo d(T)25 Beads E7499AVIAL 4 1 x 0.48 ml Not Applicable
  • E7490L     4    
      NEBNext RNA Binding Buffer (2X) E7492AAVIAL 4 1 x 28.8 ml Not Applicable
      NEBNext Wash Buffer E7493AAVIAL 4 2 x 57.6 ml Not Applicable
      Nuclease-free Water E7495AAVIAL 4 1 x 4.8 ml Not Applicable
      NEBNext Tris Buffer E7496AAVIAL 4 1 x 24 ml Not Applicable
      NEBNext Oligo d(T)25 Beads E7499AAVIAL 4 1 x 1.92 ml Not Applicable

Properties & Usage

Materials Required but not Supplied

  • 96-well 0.2 ml PCR Plates and Microseal® 'B' Adhesive Sealer (BioRad MSB-1001) or 0.2 ml RNase-free tube.
  • Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)
  • Thermal cycler or heat block
  • Bioanalyzer® (Agilent Technologies, Inc.) or similar instrument and consumables.

Application Features

Isolation of poly(A)+ RNA transcript from Total RNA for RNA library preparation and sequencing.

Protocols, Manuals & Usage

Protocols

  1. Where can I find protocols for using NEBNext® RNA Library Prep reagents?

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Usage & Guidelines

FAQs & Troubleshooting

FAQs

  1. Can I isolate poly(A)+ mRNA directly from tissue lysis using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490)?
  2. Do I need two rounds of binding?
  3. Can I use the Oligo (dT)25 Magnetic Beads (NEB# S1419S) to isolate poly(A)+ mRNA for Next Generation Sequence applications?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

This product is for research use only, not for therapeutic use, and for use only in nucleic acid purification as part of sample preparation prior to sequencing.

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