Product Class: Other

Monarch® gDNA Removal Columns

Effective May 2, 2024, the Monarch gDNA Removal Columns are now tinted orange to help distinguish from other columns.

Product Introduction

The Monarch gDNA Removal Columns:

  • Are a component of the Monarch Total RNA Miniprep Kit (NEB #T2010)
  • Bind genomic DNA during the total RNA miniprep workflow to remove it from your RNA prep
  • Contain 100 columns and 100 collection tubes
  • Are manufactured with sustainability in mind
Catalog # Size Concentration
T2017L 100.0 columns

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Product Information

Description

Monarch® gDNA Removal Columns, supplied with the Monarch Total RNA Miniprep Kit, bind genomic DNA during the total RNA miniprep workflow, removing it from your RNA preps. Columns are supplied with Monarch Collection Tubes II (NEB #T2018), and are compatible with vacuum manifolds.

 

This product is related to the following categories:
Total RNA Extraction & Purification Products,
RNA Extraction and Purification,
Nucleic Acid Purification Products,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Protocols, Manuals & Usage

Protocols

  1. Quick Protocol for Total RNA Extraction (NEB #T2010)
  2. FFPE Tissue Deparaffinization and Subsequent RNA Purification Using the Monarch Total RNA Miniprep Kit  (NEB #T2010)
  3. Total RNA Purification from Cultured Mammalian Cells using the Monarch Total RNA Miniprep Kit (NEB #T2010)
  4. Total RNA Purification from Tissues and Leukocytes using the Monarch Total RNA Miniprep Kit (NEB #T2010)
  5. Total RNA Purification from Tough-to-Lyse Samples (bacteria, yeast, plant, etc.) using the Monarch Total RNA Miniprep Kit (NEB #T2010)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Usage & Guidelines

FAQs & Troubleshooting

FAQs

  1. Can I skip the gDNA removal column step of the Monarch® Total RNA Miniprep Kit protocol?
  2. Can the genomic DNA captured on the Monarch gDNA Removal Column (NEB #T2017) be eluted and used in downstream applications?
  3. Is the Monarch® Total RNA Miniprep Kit compatible with Luna® RT-qPCR Reagents?
  4. Is the Monarch® Total RNA Miniprep Kit compatible with NEBNext® reagents for RNA Library Prep?
  5. Can the Monarch® Total RNA Miniprep Kit be used to purify RNA from bacteria?
  6. Can the Monarch® Total RNA Miniprep Kit be used to purify RNA from plants?
  7. Can the Monarch® Total RNA Miniprep Kit be used to purify RNA from yeast?
  8. What is the lowest volume in which I can elute my RNA with the Monarch Total RNA Miniprep Kit (NEB #T2010)?
  9. I noticed that in the Monarch Total RNA Miniprep Kit (NEB #T2010), the Monarch gDNA Removal Columns (NEB #T2017) are now tinted orange. Does this impact performance?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specification Change Notifications

Effective May 2, 2024, the Monarch gDNA Removal Columns are now tinted orange to help distinguish from other columns. The maximum input has been updated for cells.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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