Product Class: Other

Monarch StabiLyse™ DNA/RNA Buffer


Catalog #T2111

Product Introduction

  • DNA/RNA preservation, sample storage and lysis solution.
  • Helps to protect and stabilize RNA and DNA in samples to minimize the need for immediate sample processing.
  • Compatible with various nucleic acid extraction kits without the need for sample precipitation or reagent removal.

Bottle of T2111

Product Information

Description

The Monarch StabiLyse DNA/RNA Buffer is a dual-function reagent for nucleic acid stabilization and sample lysis. This proprietary formulation preserves the integrity of DNA and RNA in samples during storage and it also enables effective sample lysis from a wide variety of eukaryotic and prokaryotic sample types. Samples can be stored in the Monarch StabiLyse DNA/RNA Buffer at the point of harvest for short and long term without the need for immediate processing. The Monarch StabiLyse DNA/RNA Buffer is unique in that it does not require removal of this reagent or sample precipitation.

The Monarch StabiLyse DNA/RNA Buffer is ideal for performing sample homogenization and mechanical disruption. The extracted nucleic acids are suitable for a variety of downstream applications including PCR, sequencing, and more.

PROPERTIES
Recommended use Storage reagent for nucleic acid preservation and for sample lysis
Compatible with DNA and RNA extractions
Sample precipitation/reagent removal before extraction Not required –preserved samples ready for further processing and downstream applications
Sample storage recommendations At 25°C: up to 1 week
At 4°C: up to 4 weeks
At -20°C or -80°C: 4 weeks or more
Compatible with downstream applications PCR, sequencing, and more

Figure 1: Monarch StabiLyse DNA/RNA Buffer preserves DNA integrity during tissue sample storage Gel depicting preservation of DNA integrity during tissue sample storage
10 mg pieces of rat liver were stored in 100 µl Monarch StabiLyse DNA/RNA Buffer at the time of harvest and placed at room temperature, 4°C and –80°C. DNA extraction was performed using Monarch® Spin gDNA Extraction Kit (NEB #T3010) following the tissue protocol. Isolated gDNA was run on Genomic DNA ScreenTape® on Agilent® TapeStation® 4200 (Agilent Technologies). High quality DNA (> 8.0) was observed after sample storage for 1 week at room temperature, 4 weeks at 4°C and 4 weeks at –80°C.

Figure 2: Monarch StabiLyse DNA/RNA Buffer preserves RNA integrity during tissue sample storage Gel depicting preservation of RNA integrity during tissue sample storage
10 mg pieces of rat liver were stored in 200 µl Monarch StabiLyse DNA/RNA Buffer at the time of harvest and placed at room temperature, 4°C and –80°C. RNA extraction was performed using Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110) following the tissue protocol. Isolated RNA was run on RNA ScreenTape on Agilent TapeStation 4200 (Agilent Technologies). High quality RNA (> 8.0) was observed after sample storage for 1 week at room temperature, 4 weeks at 4°C and 4 weeks at –80°C.

Figure 3: Monarch StabiLyse DNA/RNA Buffer protects RNA against degradation Gel depicting protection of RNA against degradation with Monarch StabiLyse™ DNA/RNA Buffer versus PBS
Pellets of 1 million HeLa cells were resuspended in either Monarch StabiLyse DNA/RNA Buffer or Phosphate-buffered saline (PBS) and incubated at 37°C for 3 hours. RNA extraction was performed using Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110). Isolated RNA was run on RNA ScreenTape on Agilent TapeStation 4200 (Agilent Technologies). Cells resuspended in Monarch StabiLyse DNA/RNA Buffer showed intact RNA compared to PBS after heat exposure indicating protection against RNA degradation.
This product is related to the following categories:
Magnetic Bead-Based Nucleic Acid Purification,
High Molecular Weight DNA Extraction,
DNA Gel Extraction,
PCR & DNA Cleanup,
Plasmid Purification,
RNA Cleanup,
Genomic DNA Extraction & Purification,
Nucleic Acid Purification,
This product can be used in the following applications:
Nucleic Acid Purification

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Protocols, Manuals & Usage

Protocols

  1. Protocol for Fractionation of Small and Large RNA Using the Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110)
  2. Protocol for Sample Lysis of Mammalian Blood or Plasma Using the Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110)

Usage & Guidelines

FAQs & Troubleshooting

FAQs

  1. Are samples stored in DNA/RNA Protection Reagent compatible with the new version of the kit (Monarch Spin RNA Isolation Kit (Mini), NEB #T2110)?
  2. Can I use Monarch StabiLyse DNA/RNA Buffer to preserve blood samples?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Trademarks

AGILENT®, SCREENTAPE® and TAPESTATION® are registered trademarks of Agilent Technologies, Inc.

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