Product Class: Other

Monarch® Total RNA Miniprep Enzyme Pack

Product Introduction

Need more enzymes for your Monarch® Total RNA Purification Kit? No problem. Monarch Kit components are all available for purchase separately.

The Monarch Total RNA Miniprep Enzyme pack contains:

  • Monarch Proteinase K (20.8 mg)
  • Monarch Proteinase K Resuspension Buffer (1.5 ml)
  • Monarch Proteinase K Reaction Buffer (4 ml)
  • Monarch DNase I (275 units)
  • Monarch DNase I Reaction Buffer (4 ml)
  • Monarch Nuclease-free Water (1.5 ml)
Catalog # Size Concentration
T2019L 1.0 Pack

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Product Information

Description

The Monarch® Total RNA Miniprep Enzyme Pack contains lyophilized Monarch DNase I and Monarch Proteinase K and their associated reaction buffers and resuspension buffers. The enzymes in this pack are optimized for use with the Monarch Total RNA Miniprep Kit and are not recommended as substitutes for NEB's standard DNase I and Proteinase K.

This product is related to the following categories:
Total RNA Extraction & Purification Products,
RNA Extraction and Purification,
Nucleic Acid Purification Products,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • T2019L     25    
      Monarch® Proteinase K Reaction Buffer T2003-1 25 1 x 4 ml Not Applicable
      Monarch® DNase I Reaction Buffer T2005-1 25 1 x 4 ml Not Applicable
      Monarch® Nuclease-free Water T2006-21 25 1 x 1.5 ml Not Applicable
      Monarch® Proteinase K T2001-1 25 1 x 20 mg 20 mg/ml
      Monarch® Proteinase K Resuspension Buffer T2002-1 25 1 x 1.5 ml Not Applicable
      Monarch® DNase I T2004-1 25 1 x 275 units 1 units/μl

Properties & Usage

Protocols, Manuals & Usage

Protocols

  1. In-tube DNase I Treatment after Purification with the Monarch Total RNA Miniprep Kit (NEB #T2010)
  2. Quick Protocol for Total RNA Extraction (NEB #T2010)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Usage & Guidelines

FAQs & Troubleshooting

FAQs

  1. Can I skip the on-column DNase I treatment step of the Monarch® Total RNA Miniprep Kit protocol?
  2. Can I use leftover Proteinase K and DNase I from the Monarch® Total RNA Miniprep Kit for other applications?
  3. Can I use NEB Proteinase K, Molecular Biology Grade (NEB #P8107S) in conjunction with the Monarch® Total RNA Miniprep Kit?
  4. Can I use NEB DNase I (#M0303) with the Monarch Total RNA Miniprep Kit?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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