Product Class: Other

Monarch® RNA Purification Columns

Effective May 2, 2024, these Monarch RNA Purification Columns are colorless without an O-ring, whereas the Monarch gDNA Removal Columns (NEB #T2017) are now tinted orange to help distinguish from other columns

Product Introduction

Monarch RNA Purification Columns:

  • Are a component of the Monarch Total RNA Miniprep Kit (NEB #T2010)
  • Have a binding capacity of up to 100 µg of total RNA
  • Contain 100 columns and 100 collection tubes
  • Are manufactured using less plastic than other leading suppliers

Check out our Technical Note containing comprehensive insights into measuring and analyzing nucleic acids.

Catalog # Size Concentration
T2007L 100.0 columns

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Product Information

Description

Monarch® RNA Purification Columns, supplied with the Monarch Total RNA Miniprep Kit, can purify up to 100 µg of total RNA from a variety of sample inputs. Columns are supplied with Monarch Collection Tubes II, and are compatible with vacuum manifolds.

This product is related to the following categories:
Total RNA Extraction & Purification Products,
RNA Extraction and Purification,
Nucleic Acid Purification Products,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Protocols, Manuals & Usage

Protocols

  1. Quick Protocol for Total RNA Extraction (NEB #T2010)
  2. Total RNA Purification from Samples in RNAlater® using the Monarch Total RNA Miniprep Kit (NEB #T2010)
  3. Total RNA Purification from TRIzol®-extracted Samples using the Monarch Total RNA Miniprep Kit (NEB #T2010)
  4. Total RNA Purification from Samples Stored in Monarch DNA/RNA Protection Reagent using the Monarch Total RNA Miniprep Kit (NEB #T2010)
  5. FFPE Tissue Deparaffinization and Subsequent RNA Purification Using the Monarch Total RNA Miniprep Kit  (NEB #T2010)
  6. In-tube DNase I Treatment after Purification with the Monarch Total RNA Miniprep Kit (NEB #T2010)
  7. Fractionation of Small and Large RNA Using the Monarch Total RNA Miniprep Kit (NEB #T2010)
  8. RNA Reaction Cleanup using the Monarch Total RNA Miniprep Kit (NEB #T2010)
  9. Total RNA Purification from Mammalian Whole Blood Samples using the Monarch Total RNA Miniprep Kit (NEB #T2010)
  10. RNA Extraction from Saliva, Buccal Swabs, and Nasopharyngeal Swabs using the Monarch Total RNA Miniprep Kit (NEB #T2010)
  11. Automation of RNA Extraction from Saliva Using the Monarch Total RNA Miniprep Kit
  12. Total RNA Purification from Cultured Mammalian Cells using the Monarch Total RNA Miniprep Kit (NEB #T2010)
  13. Total RNA Purification from Tissues and Leukocytes using the Monarch Total RNA Miniprep Kit (NEB #T2010)
  14. Total RNA Purification from Tough-to-Lyse Samples (bacteria, yeast, plant, etc.) using the Monarch Total RNA Miniprep Kit (NEB #T2010)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Usage & Guidelines

Application Notes

FAQs & Troubleshooting

FAQs

  1. Is the Monarch® Total RNA Miniprep Kit compatible with Luna® RT-qPCR Reagents?
  2. Is the Monarch® Total RNA Miniprep Kit compatible with NEBNext® reagents for RNA Library Prep?
  3. What is the lowest volume in which I can elute my RNA with the Monarch Total RNA Miniprep Kit (NEB #T2010)?
  4. Can the Monarch® Total RNA Miniprep Kit be used to purify RNA from yeast?
  5. Can the Monarch® Total RNA Miniprep Kit be used to purify RNA from bacteria?
  6. Can the Monarch® Total RNA Miniprep Kit be used to purify RNA from plants?
  7. Can I use the Monarch® Total RNA Miniprep Kit for RNA cleanup after enzymatic manipulations?
  8. Can the Monarch Total RNA Miniprep Kit (NEB #T2010) be used to purify RNA from saliva?
  9. Can the Monarch Total RNA Miniprep Kit NEB T2010 be used to purify RNA from buccal (cheek) swabs?
  10. Can the Monarch Total RNA Miniprep Kit (NEB #T2010) be used to purify RNA from plasma or serum?
  11. Can the Monarch Total RNA Miniprep Kit (NEB #T2010) be used to purify RNA from nucleated blood (e.g. bird, fish, reptile)?
  12. Can the Monarch Total RNA Miniprep Kit be used to purify RNA from drosophila?
  13. Can the Monarch Total RNA Miniprep Kit be used to purify RNA from zebrafish embryos/larvae?
  14. I notice that I'm applying force to fit the column into a collection tube. Should I be doing this? What collection tubes are compatible with columns?
  15. Are the Monarch gDNA Removal Columns (NEB #T2017) and Monarch RNA Purification Columns (NEB #T2007) included with the Monarch Total RNA Miniprep Kit (NEB #T2010) compatible with the QIACube® System?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specification Change Notifications

Effective May 2, 2024, the maximum input has been updated for cells.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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