Product Class: Kit

NEBNext® RNA Depletion Core Reagent Set with RNA Sample Purification Beads


Catalog #E7870

Product Introduction

Introducing a new solution for customized depletion of abundant RNAs.

In RNA-seq, highly expressed transcripts with minimal biological interest, such as ribosomal RNA (rRNA) can dominate readouts and mask detection of more informative low-abundance transcripts. This challenge is amplified when working with sample types for which pre-designed RNA depletion kits are not available. The NEBNext RNA Depletion Core Reagent set provides a customized approach to deplete unwanted RNA from any organism, using probe sequences designed with the user-friendly NEBNext Custom RNA Depletion Design Tool.

The efficient RNase-H-based workflow, and close tiling of probes designed using the online tool, enables effective depletion from both low- and high-quality RNA, with a broad range of input amounts. 

  • Compatible with a broad range of input amounts: 10 ng - 1µg
  • Suitable for low-quality or high-quality RNA
  • Fast workflow: 2 hours, with less than 10 minutes hands-on time

The kit is also available without RNAClean® beads.

 

Protocols, Manuals & Usage

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Usage & Guidelines

Tools & Resources

Web Tools

FAQs & Troubleshooting

FAQs

  1. How can I design DNA probes to use with the NEBNext RNA Depletion Core Reagent Set? 
  2. Are the probes designed by the NEBNext Custom RNA Depletion Design Tool sense or antisense?
  3. Can the input sequence into the NEBNext Custom RNA Depletion Design Tool contain IUPAC Ambiguity Code?
  4. Does the NEBNext Custom RNA Depletion Design Tool check for probes targeting transcripts other than the ones provided?
  5. I have used NEBNext Custom RNA Depletion Design Tool and obtained probe sequences, what’s the next step?
  6. How do I make a working probe pool to be used with the NEBNext RNA Depletion Core Reagent Set?
  7. What’s the maximum number of probes that can be combined in a pool?
  8. Can I use this product with degraded RNA or fragmented RNA?
  9. What total RNA input should I use with the NEBNext RNA Depletion Core Reagent Set? 
  10. Why must the RNA be free of DNA?
  11. How can I determine if the RNA depletion was efficient?
  12. Does the NEBNext Custom RNA Depletion Tool check for probe redundancy or overlap between target RNA sequences entered?
  13. Can the enriched RNA resulting from the NEBNext depletion kit be used in long read sequencing applications?

Troubleshooting