NEBNext® RNA Depletion Core Reagent Set
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
E7865S |
NEBNext RNA Depletion Core Reagent Set |
6 Assays | - | Unavailable in your region | |
E7865L |
NEBNext RNA Depletion Core Reagent Set |
24 Assays | - | Unavailable in your region | |
E7865X |
NEBNext RNA Depletion Core Reagent Set |
96 Assays | - | Unavailable in your region |
NEBNext® RNA Depletion Core Reagent Set
Catalog #E7865
Product Introduction
Introducing a new solution for customized depletion of abundant RNAs.
In RNA-seq, highly expressed transcripts with minimal biological interest, such as ribosomal RNA (rRNA) can dominate readouts and mask detection of more informative low-abundance transcripts. This challenge is amplified when working with sample types for which pre-designed RNA depletion kits are not available. The NEBNext RNA Depletion Core Reagent set provides a customized approach to deplete unwanted RNA from any organism, using probe sequences designed with the user-friendly NEBNext Custom RNA Depletion Design Tool.
The efficient RNase-H-based workflow, and close tiling of probes designed using the online tool, enables effective depletion from both low- and high-quality RNA, with a broad range of input amounts.
- Compatible with a broad range of input amounts: 10 ng - 1µg
- Suitable for low-quality or high-quality RNA
- Fast workflow: 2 hours, with less than 10 minutes hands-on time
The kit is also available with RNAClean® beads.
- Product Information
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
- Other Products You May Be Interested In
Product Information
Description
RNA-seq samples can include a large dynamic range of transcript expression, and unwanted, abundant RNAs must be removed in order to achieve efficient sequencing of the RNAs of interest. While pre-designed kits are available for some sample types (e.g. human, mouse, rat, some bacteria) and abundant RNAs (e.g. rRNA, globin mRNA), a customized approach is required for rRNA removal from other sample types as well as for removal of specific non-rRNA RNAs.
Customized depletion of unwanted RNAs is enabled by use of the NEBNext RNA Depletion Core Reagent together with custom probes, following this workflow:
STEP 1: Use the online NEBNext Custom RNA Depletion Design Tool to obtain custom probe sequences, by entering the sequence of your target RNA.
STEP 2: Order ssDNA probe oligonucleotides from your trusted oligo provider.
STEP 3: Use the probes with the NEBNext Custom RNA Depletion Core Reagent Set or in combination with other NEBNext RNA Depletion Kits.
The NEBNext RNaseH-based RNA depletion workflow together with the closely-tiled custom probes designed using the online tool, allow effective depletion from both low- and high-quality RNA, with a broad range of input amounts.
The kit is also available with RNAClean® beads.
Features
- Customizable option to deplete unwanted RNA from any organism, using probe sequences designed with the NEBNext Custom RNA Depletion Design Tool.
- Compatible with a broad range of input amounts: 10 ng - 1 μg
- Suitable for low-quality or high-quality RNA
- Fast workflow: 2 hours, with less than 10 minutes hands-on time
- This product is related to the following categories:
- RNA Depletion & mRNA Enrichment Products,
- RNA Library Prep for Illumina,
- Next Generation Sequencing Library Preparation Products,
Kit Components
Kit Components
The following reagents are supplied with this product:
Properties & Usage
Related Products
Companion Products
- NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®
- NEBNext® rRNA Depletion Kit (Bacteria)
- NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat)
- NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) with RNA Sample Purification Beads
- NEBNext® Globin & rRNA Depletion Kit (Human/Mouse/Rat)
- NEBNext® Globin & rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads
- NEBNext® Ultra™ II RNA Library Prep Kit for Illumina®
Protocols, Manuals & Usage
Protocols
Manuals
Usage & Guidelines
Tools & Resources
Web Tools
FAQs & Troubleshooting
FAQs
- How can I design DNA probes to use with the NEBNext RNA Depletion Core Reagent Set?
- Are the probes designed by the NEBNext Custom RNA Depletion Design Tool sense or antisense?
- Can the input sequence into the NEBNext Custom RNA Depletion Design Tool contain IUPAC Ambiguity Code?
- Does the NEBNext Custom RNA Depletion Design Tool check for probes targeting transcripts other than the ones provided?
- I have used NEBNext Custom RNA Depletion Design Tool and obtained probe sequences, what’s the next step?
- How do I make a working probe pool to be used with the NEBNext RNA Depletion Core Reagent Set?
- What’s the maximum number of probes that can be combined in a pool?
- Can I use this product with degraded RNA or fragmented RNA?
- What total RNA input should I use with the NEBNext RNA Depletion Core Reagent Set?
- Why must the RNA be free of DNA?
- How can I determine if the RNA depletion was efficient?
- Can I use Agencourt®AMPure XP Magnetic Beads instead of RNAClean® XP Magnetic Beads/NEBNext® RNA Sample Purification Beads?
- Can I use purification methods other than NEBNext® RNA Sample Purification Beads/Agencourt® RNAClean® XP Magnetic Beads?
- Does the NEBNext Custom RNA Depletion Tool check for probe redundancy or overlap between target RNA sequences entered?
- Can the enriched RNA resulting from the NEBNext depletion kit be used in long read sequencing applications?
Troubleshooting
Citations & Technical Literature
Citations
Additional Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- E7865L_v1_10074600
- E7865S_v1_10074534
- E7865X_v1_10074616
- E7865L_v1_10089807
- E7865S_v1_10116657
- E7865X_v1_10116671
- E7865L_v1_10144899
- E7865X_v1_10152348
- E7865L_v1_10170427
- E7865S_v1_10170425
- E7865S_v1_10141978
- E7865L_v1_10141971
- E7865L_v1_10198333
- E7865S_v1_10198335
- E7865X_v1_10198337
- E7865S_v1_10208991
- E7865L_v1_10180706
- E7865S_v1_10180713
- E7865X_v1_10180720
- E7865S_v1_10223863
- E7865L_v1_10223855
- E7865X_v1_10223871
- E7865L_v1_10254474
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.NEBNext® DNase I
NEBNext® Thermostable RNase H
RNase H Reaction Buffer
DNase I Reaction Buffer
Nuclease-free Water
NEBNext Probe Hybridization Buffer
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Licenses
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information about commercial rights, please email us at gbd@neb.com While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
Other Products You May Be Interested In
-
NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®
-
NEBNext® Globin & rRNA Depletion Kit (Human/Mouse/Rat)
-
NEBNext® rRNA Depletion Kit (Bacteria)
-
NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat)
-
NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) with RNA Sample Purification Beads
-
NEBNext® Globin & rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads
-
NEBNext® Poly(A) mRNA Magnetic Isolation Module
The supporting documents available for this product can be downloaded below.