Product Class: Other

SARS-CoV-2 Positive Control (N gene)

This product will be discontinued on December 15, 2024. Please refer to the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (NEB #E2019) or the Luna® SARS-CoV-2 RT-qPCR Multiplex Assay Kit (NEB #E3019) should you wish to access this product in the future.


Catalog #N2117

Product Introduction

  • Contains full SARS-CoV-2 nucleocapsid protein (N gene)
  • Includes T7 RNA promoter sequence for generation of RNA transcripts
  • pUC19 plasmid backbone allows propagation of the plasmid in E. coli strains
  • Component of the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (NEB #E2019) and Luna® SARS-CoV-2 RT-qPCR Multiplex Assay Kit (NEB #E3019)
     
 

Product Information

Description

The SARS-CoV-2 Positive Control (N gene) is a plasmid that contains the SARS-CoV-2 N-gene (GenBank: MN908947.3). It includes a T7 RNA promoter sequence for generation of RNA transcripts, if necessary. This product is used for assay validation of RT-qPCR, LAMP, or other detection methods for SARS-CoV-2 that target the N gene. The concentration is approximately 1,000,000 copies/μl.

Please note that it is not recommended to use the Positive Control concentration as an absolute copy number for quantitative experiments. 
This product is related to the following categories:
Isothermal Amplification & Strand Displacement Products,
PCR, qPCR & Amplification Technologies Products,
This product can be used in the following applications:
Recombinase Polymerase Amplification and SIBA,
DNA Amplification, PCR & qPCR

Properties & Usage

Features

  • Provides a convenient and stable template that can be used as a positive control in SARS-CoV-2 research or diagnostic assays targeting the N gene
  • pUC19 plasmid backbone allows propagation of the plasmid in E. coli strains
 

Protocols, Manuals & Usage

Protocols

  1. SARS-CoV-2 Rapid Colorimetric LAMP Detection Assay Protocol (NEB #E2019)

Application Notes

Tools & Resources

Web Tools

FAQs & Troubleshooting

FAQs

  1. Where can I find the sequence of this positive control?
  2. Can I use the positive control as a quantitative standard?
  3. How do I make SARS-CoV-2 N gene mRNA from this positive control?
  4. Is there a polyA tail at the 3′-end of the N gene?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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