OneTaq^® Hot Start 2X Master Mix with GC Buffer

Name: One Taq ® Hot Start 2X Master Mix with GC Buffer
Brand: New England Biolabs
Price: 325.000 EUR
Availability: InStock

Product information

Code Name Size Quantity Price

M0485S

OneTaq Hot Start 2X Master Mix with GC Buffer

100 rxns ( 2 X )

Unavailable in your region

M0485L

OneTaq Hot Start 2X Master Mix with GC Buffer

500 rxns ( 2 X )

Unavailable in your region

M0485S

OneTaq Hot Start 2X Master Mix with GC Buffer

100 rxns

- 1 +

Unavailable in your region

M0485L

OneTaq Hot Start 2X Master Mix with GC Buffer

500 rxns

- 1 +

Unavailable in your region

Home PCR, qPCR & Amplification Technologies Products OneTaq^® Hot Start 2X Master Mix with GC Buffer

OneTaq^® Hot Start 2X Master Mix with GC Buffer

The ONE polymerase for your endpoint PCR needs

OneTaq^® DNA Polymerase is an optimized blend of Taq and Deep Vent® DNA polymerases for use with routine and difficult PCR experiments.

Unique hot start formulation eliminates nonspecific amplification and does not require a separate activation step
Obtain high yields with GC rich templates (>65%)
Master mix is a 2X concentrated solution containing everything needed for robust amplification

Catalog #	Concentration	Size
M0485S	2 X	100 reactions (50 µl)
M0485L	2 X	500 reactions (50 µl)
Please enter a quantity for at least one size

Product Information

OneTaq® Hot Start 2X Master Mix with GC Buffer is an optimized blend of Taq and Deep Vent® DNA Polymerases combined with an aptamer-based inhibitor. This enzyme blend is ideally suited to PCR applications from GC-rich templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The hot start nature of the enzyme offers convenience with decreased interference from primer dimers and secondary products. The convenient master mix formulation contains dNTPs, MgSO_4, buffer components and stabilizers, requiring only the addition of primers and DNA template for robust amplification.

OneTaq_ComparisonChart_0220 — Amplification of a selection of high GC human genomic DNA targets demonstrates OneTaq performance. All polymerases were cycled according to manufacturer’s recommendations, including the use of additives to enhance the amplification of targets with high GC content. Yield (dot size) and purity (color) of reaction product were quantified from triplicate reactions on a Perkin Elmer LabChip^®. A large, dark green dot represents the highest yield and purity.

Product Source

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent^® DNA Polymerase gene from Pyrococcus species GB-D.

This product is related to the following categories:: OneTaq® DNA Polymerases,; Master Mixes,; Taq DNA Polymerase

This product can be used in the following applications:: Hot Start PCR,; Multiplex PCR,; Specialty PCR,; Routine PCR,

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

M0485S

-20

	OneTaq^® Hot Start 2X Master Mix with GC Buffer	M0485SVIAL	-20	2 x 1.25 ml	2 X
	OneTaq^® High GC Enhancer	B9026AVIAL	-20	1 x 2 ml	Not Applicable

M0485L

-20

	OneTaq^® Hot Start 2X Master Mix with GC Buffer	M0485SVIAL	-20	10 x 1.25 ml	2 X
	OneTaq^® High GC Enhancer	B9026AVIAL	-20	3 x 2 ml	Not Applicable

Properties & Usage

1X Master Mix Composition

80 mM Tris-SO₄
20 mM (NH₄)₂SO₄
2 mM MgSO₄
5% Glycerol
5% DMSO
0.06% IGEPAL® CA-630
0.05% Tween® 20
0.2 mM dNTPs
25 units/ml OneTaq Hot Start DNA Polymerase
pH 9.2 @ 25°C

Heat Inactivation
No
Unit Assay Conditions
1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 15 nM primed M13 DNA.
Advantages and Features
Features
- GC-rich PCR
- High Sensitivity PCR
- High Throughput PCR
- Colony PCR
- Long PCR (up to ~6 kb genomic)
Related Products
Companion Products
Product Notes
1. OneTaq Hot Start 2X Master Mix with GC Buffer is stable for fifteen freeze-thaw cycles when stored at -20°C.
2. OneTaq Hot Start 2X Master Mix with GC Buffer is also stable for one month at 4°C, so for frequent use, an aliquot may be kept at 4°C.
3. Product specifications for individual components in the OneTaq Hot Start 2X Master Mix with GC Buffer are available separately.
References
1. Barnes,W.M. (1994). Proc. Natl. Acad. Sci. USA. 91, 2216-2220.
2. Saiki, R.K. et al. (1985). Science. 230, 1350-1354.
3. Powell, L.M. et al. (1987). Cell. 50, 831-840.

Protocols, Manuals & Usage
Tools & Resources
- Selection Charts
  DNA Polymerase Selection Chart
  
  Thermophilic DNA Polymerases
- Web Tools
  PCR Selector
  
  Tm Calculator
FAQs & Troubleshooting
- FAQs
  What ends will my PCR products have?
  
  What are Hot Start and WarmStart^® polymerases and when would I use them?
  
  How should I determine the appropriate annealing temperature for my reaction?
  
  What should the final primer concentration be in my PCR?
  
  What are the properties of this polymerase (fidelity, product ends, max amplicon, modified base incorporation, etc.)?
  
  What are the stability and storage requirements for NEB's Taq and OneTaq DNA Polymerases?
  
  Can Taq/OneTaq^® DNA Polymerases be used for nick translation?
  
  How can I optimize my PCR when using Taq DNA Polymerase?
- Troubleshooting
  PCR Troubleshooting Guide
- Tech Tips
  
  Try using a master mix with OneTaq Hot Start DNA Polymerase to achieve highly specific and sensitive PCR amplification.
Citations & Technical Literature
- Citations
  
  Product Citation Tool
- Feature Articles
  Using aptamers to control enzyme activities: Hot Start Taq and beyond
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications & Change Notifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  M0485S_L_v2
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  M0485S_L_v1_0191703
  
  M0485S_L_v1_0201708
  
  M0485S_L_v1_0211711
  
  M0485L_v1_10013984
  
  M0485L_v1_10014391
  
  M0485L_v1_10014779
  
  M0485S_v1_10014778
  
  M0485L_v1_10016113
  
  M0485S_v1_10021835
  
  M0485L_v1_10021836
  
  M0485S_v1_10034531
  
  M0485L_v1_10034532
  
  M0485L_v1_10037121
  
  M0485S_v1_10038572
  
  M0485L_v1_10046053
  
  M0485L_v1_10048408
  
  M0485S_v1_10048405
  
  M0485L_v1_10048758
  
  M0485L_v1_10051698
  
  M0485S_v1_10054118
  
  M0485S_v1_10055500
  
  M0485L_v1_10056297
  
  M0485L_v1_10061498
  
  M0485S_v1_10061497
  
  M0485L_v1_10063559
  
  M0485S_v2_10067250
  
  M0485L_v2_10068724
  
  M0485S_v2_10092093
  
  M0485S_v2_10096454
  
  M0485S_v2_10098708
  
  M0485L_v2_10092095
  
  M0485L_v2_10106321
  
  M0485L_v2_10111730
  
  M0485S_v2_10111731
  
  M0485S_v2_10120969
  
  M0485L_v2_10126937
  
  M0485S_v2_10127987
  
  M0485L_v2_10137290
  
  M0485L_v2_10150851
  
  M0485S_v2_10152167
  
  M0485L_v2_10155210
  
  M0485S_v2_10158235
  
  M0485L_v2_10158234
  
  M0485S_v2_10162336
  
  M0485L_v2_10162335
  
  M0485L_v2_10164751
  
  M0485S_v2_10165043
  
  M0485L_v2_10169401
  
  M0485S_v2_10169400
  
  M0485S_v2_10175065
  
  M0485L_v2_10174598
  
  M0485L_v2_10179971
  
  M0485L_v2_10185082
  
  M0485S_v2_10187724
  
  M0485S_v2_10202783
  
  M0485L_v2_10202806
  
  M0485S_v2_10205863
  
  M0485L_v2_10215581
  
  M0485S_v2_10221834
  
  M0485L_v2_10221835
  
  M0485S_v2_10238330
  
  M0485S_v2_10247878
  
  M0485L_v2_10249205
  
  M0485L_v2_10265331
  
  M0485L_v2_10272594
  
  M0485L_v2_10282491
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  OneTaq^® Hot Start 2X Master Mix with GC Buffer
  
  OneTaq^® High GC Enhancer
- Legal and Disclaimers
  
  Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
  Licenses
  Nucleic acid-based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the OneTaq® Hot Start 2X Master Mix with GC Buffer for Research Use Only (RUO). Commercial use of this product may require a license from New England Biolabs, Inc. For additional information or to inquire about commercial use, please contact busdev@neb.com.
  Trademarks
  NEW ENGLAND BIOLABS^®, ONETAQ^®QUICK-LOAD^® and THERMOPOL^® are registered trademarks of New England Biolabs, Inc.
  DEEP VENT^® is a trademark of New England Biolabs, Inc.
  IGEPAL^® is a registered trademark of Rhodia Operations.
  TWEEN^® is a registered trademark of Uniqema Americas LLC.

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The supporting documents available for this product can be downloaded below.

Protocol Protocol for OneTaq® Hot Start 2X Master Mix with GC Buffer (M0485)

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OneTaq® Hot Start 2X Master Mix with GC Buffer

OneTaq® Hot Start 2X Master Mix with GC Buffer

Featured Video

Product Information

Product Source

Reagents Supplied

Properties & Usage

1X Master Mix Composition

Heat Inactivation

Unit Assay Conditions

Advantages and Features

Features

Related Products

Companion Products

Product Notes

References

Protocols, Manuals & Usage

Protocols

Usage Guidelines

Application Notes

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

Troubleshooting

Tech Tips

Citations & Technical Literature

Citations

Product Citation Tool

Feature Articles

Quality, Safety & Legal

Quality Control Assays

Specifications & Change Notifications

Certificate of Analysis

Safety Data Sheets

Legal and Disclaimers

Licenses

Trademarks

Featured Videos

Other Products You May Be Interested In

OneTaq^® Hot Start 2X Master Mix with GC Buffer

OneTaq^® Hot Start 2X Master Mix with GC Buffer