Description

The Monarch Spin Columns S2B and Tubes can be used to purify up to 500 µg of nucleic acid from enzymatic reactions. The columns are designed without the use of a retaining ring, helping to ensure no buffer retention and no carryover of contaminants. Nucleic acid can be eluted in as little as 50 μl for and is ready for use in a variety of downstream applications including RT-PCR, RNA Library Prep for NGS and transfection.

Monarch columns for RNA Cleanup are also available for 10 µg (NEB #T2037) and 50 µg (NEB #T2047) binding capacities.

Properties:

This product is related to the following categories:

RNA Cleanup Products,

RNA Extraction and Purification,

Nucleic Acid Purification Products,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

Properties & Usage

Usage & Guidelines

• Avoiding Ribonuclease Contamination
• Guidelines for RNA Quantitation
• Guidelines for Working with RNA During RNA Cleanup

Application Notes

• A Practical Guide to Analyzing Nucleic Acid Concentration and Purity with Microvolume Spectrophotometers

FAQs

1. What is the maximum binding capacity of the column for RNA Cleanup Column provided with the Monarch Spin RNA Cleanup Kit?
2. What is the smallest volume of nuclease-free water that can be used for elution with the Monarch Spin Columns S1A, S2A, S2B for RNA Cleanup?
3. Can I get better recovery with the Monarch Spin RNA Cleanup Kits if I do a second elution with my eluent from the first elution?
4. What factors affect my (A₂₆₀/A₂₃₀) when using the Monarch Spin RNA Cleanup Kits?
5. What size RNA can be purified with the Monarch Spin RNA Cleanup Kit?
6. Can I use the Monarch Spin RNA Cleanup Kit to cleanup up my DNase I-treated RNA?
7. Can I use the Monarch Spin RNA Cleanup Kits to purify RNA from agarose gels?
8. Can I use the Monarch Spin RNA Cleanup Kits to cleanup RNA after a TRIzol^®/chloroform extraction?
9. Can I use the Monarch Spin RNA Cleanup Kits to cleanup my in vitro transcription (IVT) reaction?
10. Are the Monarch Spin RNA Cleanup Kits compatible with Luna RT-qPCR reagents?
11. Are the Monarch Spin RNA Cleanup Kits compatible with NEBNext reagents for RNA library prep?
12. My sample turned cloudy after adding the Monarch RNA Cleanup Binding Buffer and ethanol. Is this normal?
13. Why do I need to incubate my column for 5 minutes with elution buffer (nuclease-free water)?
14. How can I assess RNA integrity and purity?
15. Are the columns in the Monarch Spin RNA Cleanup Kits the same as those in the Monarch Total RNA Miniprep Kit (NEB #T2010)?
16. Can I purchase Monarch^® buffers and columns separately?
17. Can I do an on column DNase I treatment with the columns for RNA Cleanup?
18. Can the Monarch Spin RNA Cleanup Kits (NEB #T2030, #T2040, #T2050) also be used to purify DNA?
19. I would like to clean up  ~150 µg of RNA. Would it be better to use multiple 50 µg columns (NEB #T2040/#T2047) or a single 500 µg column (NEB #T2050/#T2057)?
20. I notice that I'm applying force to fit the column into a collection tube. Should I be doing this? What collection tubes are compatible with columns?

Troubleshooting

• Troubleshooting Guide for RNA Cleanup

Citations & Technical Literature

Quality Assurance Statement

Specifications

• T2057L_v2
• T2057L_v3
• T2057L_v4

Certificate Of Analysis

• T2057L_v1_0011806
• T2057L_v1_10023558
• T2057L_v1_10041697
• T2057L_v1_10059921
• T2057L_v1_10068608
• T2057L_v2_10099690
• T2057L_v2_10126365
• T2057L_v3_10163958
• T2057L_v3_10197192
• T2057L_v3_10237540
• T2057L_v4_10255243

Legal and Disclaimers