Product Class: Other

Monarch® Spin Columns S2B and Tubes

Effective 9/24/2024, product name changed to Monarch Spin Columns S2B and Tubes.

Product Introduction

The Monarch Spin Columns S2B and Tubes can be used to purify up to 500 µg of nuclei acid from enzymatic reactions.

  • Monarch spin columns and collection tubes designed for nucleic acid purification
  • Minimal to no buffer retention/risk of carry-over contamination
  • Made with less plastic than conventional columns from leading suppliers

 

Catalog # Size Concentration
T2057L 100.0 columns

Product Information

Description

The Monarch Spin Columns S2B and Tubes can be used to purify up to 500 µg of nucleic acid from enzymatic reactions. The columns are designed without the use of a retaining ring, helping to ensure no buffer retention and no carryover of contaminants. Nucleic acid can be eluted in as little as 50 μl for and is ready for use in a variety of downstream applications including RT-PCR, RNA Library Prep for NGS and transfection.

Monarch columns for RNA Cleanup are also available for 10 µg (NEB #T2037) and 50 µg (NEB #T2047) binding capacities.

 

Properties:

Also included as a component in:

T2050 Monarch Spin RNA Cleanup Kit (500 μg)

Product format:

Spin columns and collection tubes for nucleic acid purification

Technology and purification format:

Silica membrane-based purification technology

Provided as:

100 columns and 100 collection tubes

Suitable for:

Cleaning up nucleic acid from a variety of enzymatic reactions; Concentration of nucleic acid into small volumes

 

This product is related to the following categories:
RNA Cleanup Products,
RNA Extraction and Purification,
Nucleic Acid Purification Products,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Protocols, Manuals & Usage

Usage & Guidelines

Application Notes

FAQs & Troubleshooting

FAQs

  1. What is the maximum binding capacity of the column for RNA Cleanup Column provided with the Monarch Spin RNA Cleanup Kit?
  2. What is the smallest volume of nuclease-free water that can be used for elution with the Monarch Spin Columns S1A, S2A, S2B for RNA Cleanup?
  3. Can I get better recovery with the Monarch Spin RNA Cleanup Kits if I do a second elution with my eluent from the first elution?
  4. What factors affect my (A260/A230) when using the Monarch Spin RNA Cleanup Kits?
  5. What size RNA can be purified with the Monarch Spin RNA Cleanup Kit?
  6. Can I use the Monarch Spin RNA Cleanup Kit to cleanup up my DNase I-treated RNA?
  7. Can I use the Monarch Spin RNA Cleanup Kits to purify RNA from agarose gels?
  8. Can I use the Monarch Spin RNA Cleanup Kits to cleanup RNA after a TRIzol®/chloroform extraction?
  9. Can I use the Monarch Spin RNA Cleanup Kits to cleanup my in vitro transcription (IVT) reaction?
  10. Are the Monarch Spin RNA Cleanup Kits compatible with Luna RT-qPCR reagents?
  11. Are the Monarch Spin RNA Cleanup Kits compatible with NEBNext reagents for RNA library prep?
  12. My sample turned cloudy after adding the Monarch RNA Cleanup Binding Buffer and ethanol. Is this normal?
  13. Why do I need to incubate my column for 5 minutes with elution buffer (nuclease-free water)?
  14. How can I assess RNA integrity and purity?
  15. Are the columns in the Monarch Spin RNA Cleanup Kits the same as those in the Monarch Total RNA Miniprep Kit (NEB #T2010)?
  16. Can I purchase Monarch® buffers and columns separately?
  17. Can I do an on column DNase I treatment with the columns for RNA Cleanup?
  18. Can the Monarch Spin RNA Cleanup Kits (NEB #T2030, #T2040, #T2050) also be used to purify DNA?
  19. I would like to clean up  ~150 µg of RNA. Would it be better to use multiple 50 µg columns (NEB #T2040/#T2047) or a single 500 µg column (NEB #T2050/#T2057)?
  20. I notice that I'm applying force to fit the column into a collection tube. Should I be doing this? What collection tubes are compatible with columns?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specification Change Notifications

Effective 9/24/2024, product name changed to Monarch Spin Columns S2B and Tubes.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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