Product Class: Other

Monarch® Spin Columns S1A and Tubes

Effective September 24, 2024, product name modified to Monarch Spin Columns S1A and Tubes.

Product Introduction

The Monarch Spin Columns S1A and Tubes can be used to purify up to 10 µg of nucleic acid from enzymatic reactions.

  • Monarch spin columns and collection tubes designed for nucleic acid purification
  • Minimal to no buffer retention/risk of carry-over contamination
  • Low elution volume (≥ 5 μl, depending on the application) from unique column design
  • Up to 5 - 10 μg nucleic acid binding capacity, depending on application
  • Made with less plastic than conventional columns from leading suppliers
Catalog # Size Concentration
T2037L 100.0 columns

Product Information

Description

Monarch Spin Columns S1A and Tubes have been designed and assembled to deliver high performance for nucleic acid purification and concentration. These columns are designed without a O-ring, eliminating buffer retention and the risk of salt carry-over contamination, resulting in highly pure nucleic acid. The tapered column design allows for elution volumes as low as 5 μl for highly concentrated nucleic acid suitable for various downstream applications. Additionally, Monarch spin columns are designed with sustainability in mind, using less plastic than conventional columns without compromising performance. There columns are compatible for centrifugation and vacuum manifold protocols and are supplied with collection tubes.



Figure 1: Features of the Monarch Spin Column S1A for Nucleic Acid Purification

T1120 Column Design

NEB® Monarch’s unique column design and membrane assembly allows high-quality nucleic acid purification with low elution volume, for highly-concentrated nucleic acid for downstream applications. The column is designed and made with significantly less plastic for a reduced environmental impact, while still maintaining structural integrity and enabling high performance..

Properties

Also included as a component in: T1120 Monarch Spin DNA Gel Extraction Kit, T1130 Monarch Spin PCR & DNA Cleanup Kit (5 μg), and T2030 Monarch Spin RNA Cleanup Kit (10 µg)
Product format: Spin columns and collection tubes for nucleic acid purification
Technology and purification format: Silica membrane-based purification technology
Provided as: 100 columns and 100 collection tubes
Suitable for: Cleaning up nucleic acid from a variety of enzymatic reactions; Concentration of nucleic acid into small volumes


This product is related to the following categories:
RNA Cleanup Products,
RNA Extraction and Purification,
Nucleic Acid Purification Products,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Protocols, Manuals & Usage

Usage & Guidelines

Application Notes

FAQs & Troubleshooting

FAQs

  1. What is the maximum binding capacity of the column for RNA Cleanup Column provided with the Monarch Spin RNA Cleanup Kit?
  2. What is the smallest volume of nuclease-free water that can be used for elution with the Monarch Spin Columns S1A, S2A, S2B for RNA Cleanup?
  3. Can I get better recovery with the Monarch Spin RNA Cleanup Kits if I do a second elution with my eluent from the first elution?
  4. What factors affect my (A260/A230) when using the Monarch Spin RNA Cleanup Kits?
  5. What size RNA can be purified with the Monarch Spin RNA Cleanup Kit?
  6. Can I use the Monarch Spin RNA Cleanup Kit to cleanup up my DNase I-treated RNA?
  7. Can I use the Monarch Spin RNA Cleanup Kits to purify RNA from agarose gels?
  8. Can I use the Monarch Spin RNA Cleanup Kits to cleanup RNA after a TRIzol®/chloroform extraction?
  9. Are the Monarch Spin RNA Cleanup Kits compatible with Luna RT-qPCR reagents?
  10. Are the Monarch Spin RNA Cleanup Kits compatible with NEBNext reagents for RNA library prep?
  11. How can I assess RNA integrity and purity?
  12. Are the columns in the Monarch Spin RNA Cleanup Kits the same as those in the Monarch Total RNA Miniprep Kit (NEB #T2010)?
  13. Can I purchase Monarch® buffers and columns separately?
  14. Can I do an on column DNase I treatment with the columns for RNA Cleanup?
  15. Can the Monarch Spin RNA Cleanup Kits (NEB #T2030, #T2040, #T2050) also be used to purify DNA?
  16. Can the Monarch Spin RNA Cleanup Kits be used for RNA extraction?
  17. I notice that I'm applying force to fit the column into a collection tube. Should I be doing this? What collection tubes are compatible with columns?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specification Change Notifications

Effective September 24, 2024, product name modified to Monarch Spin Columns S1A and Tubes.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Other Products You May Be Interested In