Monarch® PCR & DNA Cleanup Kit (5 μg)
Product informationCode | Name | Size | Quantity | Price | |
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T1030G_SAMPLE |
Monarch PCR & DNA Cleanup Kit (5ug), Sample |
10 preps | - | Unavailable in your region |
Monarch® PCR & DNA Cleanup Kit (5 μg)
This product will be discontinued by October 2024 or sooner, while supplies last. A new version of this kit is now available, featuring upgraded spin columns. Migrate to the new Monarch Spin PCR and DNA Cleanup Kit (5 μg) (NEB #T1130).
- Replaced by Monarch® Spin PCR and DNA Cleanup Kit (5 μg) on December 02, 2024
Catalog #T1030
Product Introduction
Quickly and easily purify high quality DNA from PCR and other enzymatic reactions.
- Elute in as little as 6 μl
- Prevent buffer retention and salt carry-over with optimized column design
- Save time with fast, user-friendly protocols
- No need to monitor pH
- Protocol modification allows for ssDNA purification, oligonucleotide purification, and purification of other small DNA fragments
- Buffers and columns available separately
- Significantly less plastic used when compared with other kits
- Responsibly-sourced and recyclable packaging
Check out our Technical Note containing comprehensive insights into measuring and analyzing nucleic acids.
Featured Videos
View Video Library-
Monarch® PCR & DNA Cleanup Kit Protocol
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Tips for using the Monarch® PCR & DNA Cleanup Kit
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How to recycle your Monarch® Kit components
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NEB® TV Ep. 6 – Sustainability
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Quick Tips - Can I shorten the protocol for the Monarch® Cleanup?
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Quick Tips - How do I apply my elution buffer to the Monarch® nucleic acid purification column?
- Product Information
- Protocols, Manuals & Usage
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
- Other Products You May Be Interested In
Product Information
Description
The Monarch PCR & DNA Cleanup Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from PCR and other enzymatic reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging. The protocol can also be modified to enable the purification of smaller DNA fragments, including oligonucleotides and ssDNA.
View our videos on protocols, tips, and recycling Monarch.
APPLICATIONS | |
PCR cleanup | DNA from PCR reactions can be purified after amplification to remove polymerases, primers, detergents, dNTPs, etc. |
Enzymatic reaction cleanup | Restriction enzymes and modifying enzymes such as ligases, kinases, nucleases, phosphatases are efficiently removed, allowing for effective desalting and concentration of the DNA sample. |
cDNA cleanup | DNA/RNA complexes can be purified post-reverse transcription/amplification to enable removal of the RT and polymerase as well as nucleotides. |
Labeling cleanup | Unincorporated radiolabeled or fluorescently labeled nucleotides can be removed from the DNA substrate |
Plasmid cleanup | Plasmid preps from unknown sources may contain inhibitors and unwanted contaminants. Purification and concentration can be easily achieved using this kit. |
Oligonucleotide cleanup | ssDNA oligonucleotides (≥ 18 nt) and dsDNA fragments (≥ 15 bp) can be purified using the Oligonucleotide Cleanup Protocol. |
Specifications
DNA Sample Type: | DNA from PCR and other enzymatic reactions (e.g., restriction digests, kinase reactions, ligations). ssDNA or dsDNA oligonucleotides from enzymatic reactions can also be purified using the Oligonucleotide Cleanup Protocol. |
Binding Capacity: | up to 5 μg |
DNA Size Range: | ~50 bp to 25 kb DNA ≥ 15 bp to 25 kb (dsDNA) and DNA ≥ 18 nt to 10 kb (ssDNA) can also be purified using the Oligonucleotide Cleanup Protocol. |
Typical Recovery: |
DNA (50 bp to 10 kb): 70–90% |
Elution Volume: | ≥ 6 μl |
Purity: | A260/280 > 1.8 and A260/230 > 1.8 |
Protocol Time: | 5 minutes of spin and incubation time |
Compatible Downstream Applications: |
ligation, restriction digestion, labeling and other enzymatic manipulations, library construction and DNA sequencing. |
- This product is related to the following categories:
- DNA Cleanup Products,
- Nucleic Acid Purification Products,
- This product can be used in the following applications:
- PCR & Reaction Cleanup,
- Nucleic Acid Purification
Properties & Usage
Features
- Elute in as little as 6 μl
- Prevent buffer retention and salt carry-over with optimized column design
- Save time with fast, user-friendly protocols
- No need to monitor pH
- Buffers and columns available separately
- Responsibly-sourced and recyclable packaging
- Significantly less plastic used when compared with other kits
Related Products
Companion Products
- Gel Loading Dye, Purple (6X)
- Gel Loading Dye, Purple (6X), no SDS
- Quick-Load® Purple 1 kb DNA Ladder
- Quick-Load® Purple 100 bp DNA Ladder
- Quick-Load® Purple 1 Kb Plus DNA Ladder
- T4 DNA Ligase
- Blunt/TA Ligase Master Mix
- Instant Sticky-end Ligase Master Mix
- Monarch® Plasmid Miniprep Kit
- Monarch® DNA Gel Extraction Kit
Materials Sold Separately
Product Notes
- The kit should be stored at room temperature. Always keep buffer bottles tightly closed and keep columns sealed in the enclosed zip-lock bag. For information regarding the composition of buffers, please consult the Safety Data Sheets. Proper laboratory safety practices should be employed, including the use of lab coats, gloves and eye protection.
Protocols, Manuals & Usage
Protocols
Manuals
Usage & Guidelines
Application Notes
FAQs & Troubleshooting
FAQs
- Can I purchase Monarch® buffers and columns separately?
- Can I use water to elute the DNA when using the Monarch Kits?
- What is the smallest volume of elution buffer that can be used with the Monarch DNA Cleanup Column?
- What is the composition of each buffer provided with the Monarch PCR & DNA Cleanup Kit (5 μg)?
- What is the maximum binding capacity of the Monarch DNA Cleanup Column provided with the Monarch PCR & DNA Cleanup Kit (5 μg)?
- What factors affect my (A260/A230)?
- What size primers can be effectively removed from a PCR reaction?
- Can the Monarch PCR & DNA Cleanup Kit (5 μg) be used to purify RNA?
- Do you have any recommendations for purification of ssDNA?
- Are the columns in the Monarch PCR & DNA Cleanup Kit (5 μg) the same as the ones in the Monarch DNA Gel Extraction Kit?
- What size of DNA can be purified with the Monarch DNA Cleanup Columns?
- After purification, I see a faint additional band running below the expected size on a gel. What happened?
- Are Monarch spin columns compatible with Vacuum Manifolds?
- Can I use the Monarch DNA & PCR Cleanup Kit to purify oligonucleotides and other short DNA fragments?
- If I need to clean up more than 5 μg of DNA, is there another higher capacity column alternative to the columns included in the Monarch PCR & DNA Cleanup kit (NEB #T1030)?
- I notice that I'm applying force to fit the column into a collection tube. Should I be doing this? What collection tubes are compatible with columns?
Troubleshooting
Low DNA Yield
- Reagents added incorrectly. Check protocol to ensure correct buffer reconstitution, order of addition for buffers, and proper handling of column flow-through and eluents.
- Incomplete elution during prep. Ensure the DNA Elution Buffer is delivered directly to the center of the column so that the matrix is completely covered and elution is efficient. Larger elution volumes and longer incubation times can increase yield of DNA off the column at the cost of dilution of the sample and increased processing times. For typical fragments below 10 kb, the recommended elution volumes and incubation times should be sufficient, unless the maximal yield is desired. For the purification of larger fragments, heating the DNA Elution Buffer to 50°C prior to eluting and extending the incubation time after buffer addition to 5 minutes can improve yield. Additionally, multiple rounds of elution can be employed to increase the amount of DNA eluted, at the expense of dilution of the sample.
- Ethanol has been carried-over. Ensure final wash spin time is 1 minute to ensure complete removal of the wash buffer from the column, and be careful when transferring the column to a new tube for elution step to ensure column tip does not contact column flow-through.
- Trace amounts of salts that produce low OD260/230 ratios can also be carried over during the elution step. Be careful when transferring column to new tube for elution step to ensure the column tip does not contact column flow-through.
Tech Tips
Learn how to isolate DNA from your enzymatic reactions, including PCR, using the Monarch PCR & DNA Cleanup Kit (5 µg).
Optimize your DNA isolation from PCR and other enzymatic reactions with our quick tips for using the Monarch PCR & DNA Cleanup Kit.
Learn how you can easily recycle all of the components in your Monarch Kits.
Citations & Technical Literature
Citations
Additional Citations
- Fomenkov, A., Vincze, T., Mersha, F., Roberts, R.J. (2018) Complete genome sequence and methylome analysis of Bacillus caldolyticus NEB414. Genome Announ.; 6 (6), e01605-17. PubMedID: 29439055 , DOI: 10.1128/genomeA.01605-17
- Bornikoel J, Staiger J, Madlung J, Forchhammer K, Maldener I. (2018) LytM factor Alr3353 affects filament morphology and cell-cell communication in the multicellular cyanobacterium Anabaena sp. PCC 7120 Mol Microbiol; PubMedID: 29437253
- Wei, H., Yan, B., Gagneur, J., Conradt, B. (2017) Caenorhabditis elegans CES-1 snail represses Pig-1 MELK expression to control asymmetric cell division. Genetics; 206(4), PubMedID: 28652378
- James L. Dimond,Sanoosh K. GamblewoodSteven B. Roberts (2017) Genetic and epigenetic insight into morphospecies in a reef coral. Mol Ecol; PubMedID: 28753237
- Olga De Castro , Maria Comparone, Antonietta Di Maio, Emanuele Del Guacchio, Bruno Menale, Jacopo Troisi, Francesco Aliberti, Marco Trifuoggi , Marco Guida (2017) What is in your cup of tea? DNA Verity Test to characterize black and green commercial teas. PLOS One; PubMedID: 28542606
- Su M, Kirchner A, Stazzoni S, Müller M, Wagner M, Schröder A, Carell T. (2016) 5-Formylcytosine Could Be a Semipermanent Base in Specific Genome Sites. Angew Chem Int Ed Engl; PubMedID: 27561097
- Anismrita Lahon, Ravi P. Arya, Alexander R. Kneubehl, Megan B. Vogt, Natalie J. M. Dailey Garnes, and Rebecca Rico-Hesse,* (2016) Characterization of a Zika Virus Isolate from Colombia. PLoS One; PubMedID: 5031432
Publications
- Wei, H., Yan, B., Gagneur, J., Conradt, B. (2017) Caenorhabditis elegans CES-1 snail represses Pig-1 MELK expression to control asymmetric cell division. Genetics; 206(4), PubMedID: 28652378
- Vladimir Potapov, Jennifer L. Ong. (2017) Examining Sources of Error in PCR by Single-Molecule Sequencing. PLOS One; PubMedID: 28683110
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- T1030G_v1_0031604
- T1030S_L_v1_0071711
- T1030G_v1_0091807
- T1030L_v1_0091807
- T1030S_v1_0091807
- T1030S_v1_0101810
- T1030G_v1_0101810
- T1030L_v1_0101810
- T1030G_v1_10020891
- T1030L_v1_10020889
- T1030S_v1_10020890
- T1030G_v2_10029804
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- T1030L_v2_10029802
- T1030G_v2_0101810
- T1030G_v2_10040569
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- T1030S_v2_10040568
- T1030G_v2_10040693
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- T1030L_v2_10132576
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- T1030G_v2_10135038
- T1030L_v2_10135039
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- T1030G_v2_10166578
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- T1030L_v2_10175903
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- T1030L_v2_10208654
- T1030S_v2_10208655
- T1030G_v2_10208653
- T1030S_v2_10240883
- T1030L_v2_10240880
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
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The supporting documents available for this product can be downloaded below.