Product Class: Other

Monarch® DNA Cleanup Columns (5 μg)

This product will be discontinued by November 2024 or sooner, while supplies last.

A new version of this product is now available. Migrate to the Monarch® Spin Columns S1A and Tubes (NEB #T2037L).

Product Introduction

Need more columns? No problem! Monarch buffers and columns are all sold separately for your convenience. Monarch DNA Cleanup columns can be used to purify up to 5 μg of DNA from enzymatic reactions and agarose gels. 

Check out our Technical Note containing comprehensive insights into measuring and analyzing nucleic acids.

Catalog # Size Concentration
T1034L 100.0 columns

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Product Information

Description

Highlights


  • No buffer retention/risk of carryover contamination
  • Low elution volumes (≥ 6 μl)
  • Snug fit into collection tubes for easy handling
  • Made with less plastic than conventional columns
Monarch DNA Cleanup Columns (5 μg), supplied with both the Monarch DNA Gel Extraction Kit and the Monarch PCR & DNA Cleanup Kit (5 μg), have been custom designed to deliver excellent performance for the purification of DNA from agarose gels and enzymatic reactions, including PCR. Monarch columns are designed without a frit (which is commonly used in purification columns to hold the membrane in place), eliminating buffer retention and the risk of carryover contamination. The tapered design of the DNA Cleanup Column enables elution in as little as 6 μl. Monarch columns are suitable for use with centrifugation and vacuum purification protocols. The columns fit snugly into the collection tubes to enable easy handling. Additionally, Monarch columns use less plastic than conventional columns, reducing their environmental footprint without compromising performance. Stand-alone Monarch columns are supplied with collection tubes.
Monarch DNA Cleanup Column Design 
Monarch columns are designed for performance
Monarch columns are designed without a frit, which eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification.

Learn More About Monarch
This product is related to the following categories:
DNA Gel Extraction Products,
DNA Cleanup and Gel Extraction Products,
DNA Cleanup Products,
Nucleic Acid Purification Products,
This product can be used in the following applications:
PCR & Reaction Cleanup,
Nucleic Acid Purification

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • T1034L     25    
      Monarch® DNA Cleanup Columns (5 μg) T1034-2 25 2 x 50 columns Not Applicable
      Monarch® Collection Tubes (2 ml) T1018-2 25 2 x 50 tubes Not Applicable

Properties & Usage

Binding Capacity

5 μg

Protocols, Manuals & Usage

Protocols

  1. Monarch® DNA Gel Extraction Kit Protocol (NEB #T1020)
  2. Protocol for DNA Cleanup and Concentration Using the Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB #T1030)
  3. Oligonucleotide Cleanup Using Monarch® PCR & DNA Cleanup Kit (5 μg) Protocol (NEB #T1030)

Usage & Guidelines

Application Notes

FAQs & Troubleshooting

FAQs

  1. Can I use the Monarch DNA & PCR Cleanup Kit to purify oligonucleotides and other short DNA fragments?
  2. If I need to clean up more than 5 μg of DNA, is there another higher capacity column alternative to the columns included in the Monarch PCR & DNA Cleanup kit (NEB #T1030)?
  3. I notice that I'm applying force to fit the column into a collection tube. Should I be doing this? What collection tubes are compatible with columns?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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