Product Class: Other

Streptavidin Magnetic Beads

Streptavidin Magnetic Beads have been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10249776. Learn more.

Product Introduction

Streptavidin Magnetic Beads are 1 µm superparamagnetic particles covalently coupled to a highly pure form of streptavidin. The beads can be used to capture biotin labeled substrates including antigens, antibodies and nucleic acids.

  • The strength of the biotin-streptavidin interaction, an association constant (Ka) of 1015 M-1, coupled with the low non-specific binding of streptavidin permits captured substrates to be useful as ligands in subsequent experiments including mRNA isolation, nucleic acid enrichments and the capture of primary or secondary antibodies
  • Other applications include 5-hmC detection and analysis, methylated DNA analysis, and solution-phase panning in phage display experiments
  • Binding capacity:  ≥30 µg biotinylated antibody per mg and >500 pmol of single-stranded 25 bp biotinylated oligionucleotide per mg
  • High binding capacity and low non-specific binding of proteins make this bead ideally suited for protein isolation applications
  • Quick, small-scale purification of biotinylated proteins affords higher throughput and efficiency
Catalog # Size Concentration
S1420S 5.0 ml 4 mg/ml

Product Information

Description

Streptavidin Magnetic Beads are 1 μm superparamagnetic particles covalently coupled to a highly pure form of streptavidin. The beads can be used to capture biotin labeled substrates including antigens, antibodies and nucleic acids (1,2). The strength of the biotin/streptavidin interaction, an association constant (Ka) of 1015 M-1 coupled with the low nonspecific binding of streptavidin permits captured substrates to be useful as ligands in subsequent experiments including mRNA isolation and the capture of primary or secondary antibodies.

For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact custom@neb.com for further information.

This product can be used in the following applications:
5-hmC Detection & Analysis,
Phage Display

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Storage Buffer

0.1% Recombinant Albumin
0.05% Tween® 20
0.05% NaN3
1X PBS
pH 7.4 @ 25°C

Support Matrix

1 µM nonporous superparamagnetic microparticle

Binding Capacity

The beads will bind greater than 1000 pmol of free biotin per mg and greater than 500 pmol of single-stranded 25 bp biotinylated oligionucleotide per mg.

Shipping Notes

  • 4°C

Product Notes

  1. Beads will bind greater than 1000 pmoles of free biotin per mg. Beads will bind greater than 500 pmoles of single-stranded 20 bp biotin oligonucleotide per mg.
  2. Support Matrix: 1 µM nonporous superparamagnetic microparticle.

References

  1. He, M. and Taussig, M. (1997). Nucl. Acids Res.. 25, 5132-5134.
  2. Cuddy, K. et al. (1993). Nucl. Acids Res.. 21, 2281.

Protocols, Manuals & Usage

Protocols

  1. mRNA Isolation using Streptavidin Magnetic Beads (NEB #S1420 and NEB #S1421)
  2. Phage Display: Solution-phase Panning with Affinity Bead Capture
  3. Protocol: Binding biotinylated nucleic acids, antibodies, or proteins to Streptavidin Magnetic Beads for Pull-down Experiments (NEB #S1420)

FAQs & Troubleshooting

FAQs

  1. What is the difference between S1420 and S1421?
  2. Do I need to block the beads or are they pre-blocked?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specification Change Notifications

Streptavidin Magnetic Beads have been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10249776. For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin).

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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