Product Class: Other

Hydrophilic Streptavidin Magnetic Beads

Product Introduction

Hydrophilic Streptavidin Magnetic Beads are 2-3 µm superparamagnetic particles covalently coupled to a highly pure form of streptavidin. The beads can be used to capture biotin labeled substrates including antigens, antibodies and nucleic acids.

  • The strength of the biotin-streptavidin interaction, an association constant (Ka) of 1015 M-1 coupled with the low non-specific binding of streptavidin permits captured substrates to be useful as ligands in subsequent experiments including mRNA isolation and the capture of primary or secondary antibodies
  • Binding capacity: ≥30 µg biotinylated antibody per mg and > 400 pmol of single-stranded 25 bp biotinylated oligonucleotide per mg
  • Quick, small-scale purification of biotinylated oligonucleotides affords higher throughput and efficiency
Catalog # Size Concentration
S1421S 5.0 ml 4 mg/ml

Product Information

Description

Hydrophilic Streptavidin Magnetic Beads are 2 µm superparamagnetic particles covalently coupled to a highly pure form of streptavidin. The beads can be used to capture biotin labeled substrates including antigens, antibodies and nucleic acids (1,2). The strength of the biotin-streptavidin interaction, an association constant (Ka) of 1015 M-1 coupled with the low non-specific binding of streptavidin permits captured substrates to be useful as ligands in subsequent experiments including mRNA isolation and the capture of primary or secondary antibodies (3,4).

Beads are supplied as a 4 mg/ml suspension in a phosphate buffer (PBS) (pH 7.4) containing 0.1% BSA, 0.05% NaN3 and 0.05% Tween® -20.

For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact custom@neb.com for further information.

This product is related to the following categories:
DNA Methylation Analysis,
RNA Modification,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Storage Buffer

0.1% BSA
0.05% Tween® 20
0.05% NaN3
1X PBS
pH 7.4 @ 25°C

Support Matrix

2 µM non-porous superparamagnetic microparticles

Binding Capacity

The beads will bind greater than 800 pmol of free biotin per mg and greater then 400 pmol of single-stranded 25 bp biotinylated oligonucleotide per mg.

Product Notes

  1. Beads will bind greater than 800 pmol of free biotin per mg and greater than 400 pmol of single-stranded 20 bp biotinylated oligonucleotide per mg of Hydrophilic Streptavidin Magnetic Beads.

References

  1. Chung, S. et al. (2005). J. Biol. Chem.. 280, 4578.
  2. Tang, B. et al. (2003). Genome. 46, 833--840.
  3. Zhang, X. et al. (2006). J. Clin. Invest.. 116, 3050-3059.
  4. Fernandez-Cabezudo, M.J. et al. (2004). Internat. Immunol.. 16, 1215-1223.

Protocols, Manuals & Usage

Protocols

  1. Phage Display: Solution-phase Panning with Affinity Bead Capture
  2. mRNA Isolation using Streptavidin Magnetic Beads (NEB #S1420 and NEB #S1421)
  3. Binding biotinylated nucleic acids, antibodies, or proteins to Hydrophilic Streptavidin Magnetic Beads (NEB #S1421) for Pull-Down experiments

FAQs & Troubleshooting

FAQs

  1. What is the difference between S1420 and S1421?
  2. Do I need to block the beads or are they pre-blocked?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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