Product Class: Other

Protein A Magnetic Beads

Product Introduction

Protein A Magnetic Beads are an affinity matrix for the small-scale isolation and purification of immunoglobulins. Specifically, the beads consist of a truncated form of recombinant Protein A that is covalently coupled to a nonporous superparamagnetic particle. 

  • Exhibits high affinity for subclasses of IgG from many species including human, rabbit and mouse
  • The protein is coupled through a linkage that is stable and leak resistant over a wide pH range. This permits the immunomagnetic purification of IgGs from ascites, serum or cell culture supernatants 
  • The matrix can be regenerated without loss of binding capacity
  • Can be used to immunoprecipitate target proteins from crude cell lysates using a selected primary antibody. In addition, specific antibodies can be chemically cross-linked to the Protein A coated surface to create a reusable immunoprecipitation bead, thereby avoiding the co-elution of antibody with the target antigen 
  • Binding capacity: 1 ml of Protein A Magnetic Beads binds > 280 µg of Human IgG
  • Quick, small-scale purification of many subclasses of IgGs affords higher throughput and efficiency
 
Catalog # Size Concentration
S1425S 1.0 ml

Product Information

Description

Protein A Magnetic Beads are an affinity matrix for the small-scale isolation and purification of immunoglobulins. A truncated form of recombinant Protein A is covalently coupled to a nonporous superparamagnetic particle. Protein A exhibits high affinity for subclasses of IgG from many species including human, rabbit and mouse (1). The protein is coupled through a linkage that is stable and leak resistant over a wide pH range. This permits the immunomagnetic purification of IgGs from ascites, serum or cell culture supernatants; the matrix can then be regenerated without loss of binding capacity. Protein A Magnetic Beads can be used to immunoprecipitate target proteins from crude cell lysates using selected primary antibody. In addition, specific antibodies can be chemically cross-linked to the Protein A coated surface to create a reusable immunoprecipitation bead, avoiding the co-elution of antibody with target antigen (2,3).

For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact custom@neb.com for further information.


This product is related to the following categories:
Enrichment,
Chromatin Analysis,
Magnetic Beads and Racks Products,
DNA Methylation Analysis,
This product can be used in the following applications:
ChIP,
DNA Enrichment,
Antibodies,
Pull Down Assays,
5-hmC Detection & Analysis,
Phage Display,
High-throughput cloning and automation solutions

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Storage Buffer

0.05% NaN3
0.1% BSA
0.05% Tween® 20
1X PBS
pH 7.4 @ 25°C

Support Matrix

2 µm nonporous superparamagnetic microparticles

Binding Capacity

1 ml of Protein A Magnetic Beads binds > 280 µg of Human IgG.

Can be Regenerated

Yes

Product Notes

  1. Support Matrix: 2 µm nonporous superparamagnetic microparticles. (1 ml of Protein A Magnetic Beads will bind > 280 µg of Human IgG.)
  2. Affinity of Protein A/G for IgG Types from Different Species

References

  1. Harlow, E. and Lane D. (1988). Bacterial Cell Wall Proteins that Bind Antibodies. Antibodies: A Laboratory Manual. 615-619.
  2. Schneider, C., Newman, R.A. et al. (1982). J. Biol. Chem.. 257, 10766.
  3. Sisson, T.H. and Castor, C.W. (1990). Immunol. Methods. 127, 215.

Protocols, Manuals & Usage

Protocols

  1. Purification of IgG using Protein A/G Magnetic Beads
  2. Immunoprecipitation using Protein A/G Magnetic Beads
  3. Cross-linking of IgG to Protein A or G Beads
  4. Phage Display: Solution-phase Panning with Affinity Bead Capture

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. Do I need to block the beads or are they pre-blocked?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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