Product Class: Other

Amylose Magnetic Beads

Product Introduction

An affinity matrix for the small-scale isolation and purification of maltose binding protein (MBP)-fusion proteins. 

  • Amylose is covalently coupled to a superparamagnetic particle through a linkage that is stable and leak resistant over a wide pH range, thereby permitting the isolation of MBP-fusion proteins from cell culture supernatants
  • Immobilized fusion proteins can be used in subsequent experiments to capture (pull down) target proteins from crude cell lysates that may interact with the immobilized fusion protein 
  • Binding capacity of 10 µg MBP5*-paromyosin ΔSal fusion protein/mg amylose magnetic beads
  • Quick, small-scale purification of MBP-fusion proteins affords higher throughput and efficiency
Catalog # Size Concentration
E8035S 25.0 mg 10 mg/ml

Product Information

Description

An affinity matrix for the small-scale isolation and purification of MBP-fusion proteins. Amylose is covalently coupled to a paramagnetic particle through a linkage that is stable and leak resistant over a wide pH range. This permits the isolation of MBP-fusion proteins from cell culture supernatants. Immobilized fusion proteins can be used in subsequent experiments to capture target proteins from crude cell lysates that may interact with the immobilized fusion protein.

Supplied as a 10 mg/ml suspension in water containing 20% ethanol.

DNASU is a central repository for plasmid clones and collections that may also be helpful.

This product is related to the following categories:
Amylose Purification (MBP-tag),
Affinity Purification Products,
Magnetic Beads and Racks Products,
Protein Purification Products,
This product can be used in the following applications:
MBP Affinity Tag,
Pull Down Assays,
Affinity Purification & Expression Tags,
Protein Purification,
Phage Display,
High-throughput cloning and automation solutions

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Support Matrix

10 μm superparamagnetic microparticles

Binding Capacity

10 ug MBP5* paromysin Δ sal / mg amylose magnetic beads

Storage Notes

  • Do not freeze

Shipping Notes

  • 4°C

Product Notes

  1. Visual appearance of bead bed volume may vary from lot to lot; however, this has no impact on functional performance or bead binding capacity. The mass concentration of the bead suspension is normalized to 10 mg/ml and assayed as part of lot release criteria.

Protocols, Manuals & Usage

Protocols

  1. Isolation of MBP-fusion protein using Amylose Magnetic Beads

Tools & Resources

Selection Charts

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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