Product Class: Other

N1-Methyl-Pseudouridine-5’-Triphosphate (N1-Methyl-Pseudo-UTP)


Catalog #N0431

Product Introduction

  • Supplied in sodium salt form, pH 7.5.
  • Modified NTPs are commonly used for reduction of immunogenicity for in vitro transcription RNA.
  • No detectable RNase, DNase, phosphatase or protease contaminating activities.
  • ≥95% purity, as determined by HPLC.
  • Optimal activity and stability for up to 24 months. 
  • NTPs are also available as Ribonucleotide Solution Set (NEB #N0450) or Ribonucleotide Solution Mix (NEB #N0466).
  • Getting ready to scale up RNA synthesis? Download our new technical note “Scaling of High-Yield In vitro Transcription Reactions for Linear Increase of RNA Production” for a generalized set of recommendations for synthesizing high yields of RNA.
 

Product Information

Description

mRNA has emerged as a potent modality for therapeutics. Incorporation of chemically modified ribonucleotides can improve the utility of mRNA by increasing stability, reducing immunogenicity, and enhancing translatability.

 

 

 
This product is related to the following categories:
RNA Synthesis and Modification,
Nucleotide Solutions Products,
RNA Modification,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • N0431S     -20    
      N1-Methyl-Pseudouridine-5’-Triphosphate (N1-Methyl-Pseudo-UTP) N0431SVIAL -20 1 x 0.1 ml 100 mM

Properties & Usage

References

  1. Karikó, M. Buckstein, H. Ni, D. Weissman (2005). Immunity. 23(2), 165-175,
  2. Andries, S. McCafferty, S. C. De Smedt, R. Weiss, N. N. Sanders, T. Kitada, J (2015). Controlled Release. 217, 337, DOI: https://doi.org/10.1016/j.jconrel.2015.08.051,
  3. Li, X. Luo, Y. Dong (2016). Bioconjugate Chem. 27, 3, 849-853, DOI: https://doi.org/10.1021/acs.bioconjchem.6b00090,

FAQs & Troubleshooting

FAQs

  1. Can modified ribonucleotides be used with HiScribe® T7 High Yield RNA Synthesis Kit (NEB #E2040)?
  2. Can I completely substitute a modified NTP for the canonical NTP counterpart? Will complete substitution impact yield?
  3. Can I use more than one modified NTP in the same IVT? Will this impact yield?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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