Product Class: Kit

Ribonucleotide Solution Set

Product Introduction

Catalog # Size Concentration
N0450S 10.0 µmol
N0450L 50.0 µmol

Featured Videos

View Video Library
  • RNase_Contamination_Anim_720x405

    Avoiding RNase Contamination

Product Information

Description

The Ribonucleotide Solution Set (NTP Set) consists of four separate solutions of ATP, GTP, CTP and UTP, pH 7.5 as sodium salts. The NTPs are also available as a Ribonucleotide Solution Mix (NEB #N0466).
This product is related to the following categories:
Nucleotide Solutions Products,
RNA Synthesis In vitro Transcription (IVT),
This product can be used in the following applications:
PCR

Kit Components

Kit Components

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • N0450S     -20    
      ATP N0451AVIAL -20 1 x 0.1 ml 100 mM
      GTP  N0452AVIAL -20 1 x 0.1 ml 100 mM
      UTP  N0453AVIAL -20 1 x 0.1 ml 100 mM
      CTP  N0454AVIAL -20 1 x 0.1 ml 100 mM
  • N0450L     -20    
      ATP N0451AAVIAL -20 1 x 0.5 ml 100 mM
      GTP  N0452AAVIAL -20 1 x 0.5 ml 100 mM
      UTP  N0453AAVIAL -20 1 x 0.5 ml 100 mM
      CTP  N0454AAVIAL -20 1 x 0.5 ml 100 mM

Properties & Usage

Application Features

In vitro transcription
RNA tailing by Poly(A) and Poly(U) Polymerases
Substrates or cofactors for many enzymes

Product Notes

  1. Store at -20°C. Avoid multiple freeze/thaw cycles. Dilute with nuclease-free water when necessary. NTPs are used with many RNA products from NEB.

Protocols, Manuals & Usage

Application Notes

FAQs & Troubleshooting

Tech Tips

It is important to vortex the tubes prior to use due to high concentration of the nucleotides.

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Other Products You May Be Interested In