Faustovirus Capping Enzyme
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
M2081S |
Faustovirus Capping Enzyme |
500 units ( 25000 units/ml ) | - | Unavailable in your region | |
M2081L |
Faustovirus Capping Enzyme |
2500 units ( 25000 units/ml ) | - | Unavailable in your region |
Faustovirus Capping Enzyme
GMP-grade coming soon.
Product Introduction
- Faustovirus Capping Enzyme adds the m7G-cap (Cap-0) to the 5′ end of triphosphorylated and diphosphorylated RNA
- Experience improved capping efficiency, even on difficult substrates
- Achieve robust capping with less enzyme
- Set up reactions under a broad temperature range, for added flexibility
- Choose as an alternative to Vaccinia Capping System, with minimal optimization required
- Enable one-pot Cap-1 synthesis, as FCE is compatible with mRNA Cap 2′-O-Methyltransferase
- Benefit from no licensing fees from NEB for the use of FCE
- Getting ready to scale up RNA synthesis? Download our new technical note “Scaling of High-Yield In vitro Transcription Reactions for Linear Increase of RNA Production” for a generalized set of recommendations for synthesizing high yields of RNA.
Catalog # | Size | Concentration |
---|---|---|
M2081S | 500.0 units | 25000 units/ml |
M2081L | 2500.0 units | 25000 units/ml |
- Product Information
- Protocols, Manuals & Usage
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
Product Information
Description
Faustovirus Capping Enzyme (FCE) catalyzes the addition of N7-methylguanosine cap (m7G) to the 5′ end of triphosphorylated and diphosphorylated transcripts, producing Cap-0 RNA(1). FCE is a single-subunit enzyme that combines the three activities necessary to produce the Cap-0 structure- triphosphatase, guanylyltransferase, and (guanine-N7)-methyltransferase. Installation of Cap-0 is a key step in eukaryotic mRNA maturation along with 2′-O-methylation at position 1 (Cap-1) and polyadenosine (poly(A)) tailing. The Cap-0 structure also promotes RNA stability(2) and prevents inadvertent activation of innate immune responses triggered by triphosphorylated RNA(3).FCE retains significant capping activity at low temperatures and tolerates reaction temperatures up to 55°C. In many cases, 1 µl of FCE (25 units) can cap over 100 µg of RNA in 1 hour at 37°C. GTP and S-adenosylmethionine (SAM) are required for capping activity and are included with the enzyme.
Source: An E. coli strain that carries a plasmid encoding the Faustovirus Capping Enzyme with a C-terminal His-tag.
- This product is related to the following categories:
Reagents Supplied
Reagents Supplied
The following reagents are supplied with this product:
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Properties & Usage
Unit Definition
One unit of Faustovirus Capping Enzyme is defined as the amount of enzyme required to convert 75 pmol of a 20-mer transcript to Cap-0 RNA in 30 minutes at 37°C.Reaction Conditions
1X Capping Buffer
Supplement with 0.5 mM GTP and 0.1 mM S-adenosylmethionine (SAM)
Incubate at 37°C
1X Capping Buffer
50 mM Tris-HCl
5 mM KCl
1 mM MgCl2
1 mM DTT
(pH 8 @ 25°C)
Heat Inactivation
70°C for 10 minutesAddition of EDTA to 5 mM is recommended to avoid RNA hydrolysis.
Features
- Experience improved capping efficiency, even on difficult substrates
- Achieve robust capping with less enzyme
- Set up reactions under a broad temperature range, for added flexibility
Related Products
Companion Products
References
- Ramanathan, A. et al. (2016). Nucleic Acids Res . 44 (16), 7511–7526.
- Furuichi, Y., LaFiandra, A. & Shatkin, A. (1977). Nature. 266, 235-239.
- Schlee, M., Hartmann, G. (2016). Nat Rev Immunol . 16, 568-580.
Protocols, Manuals & Usage
Protocols
Application Notes
FAQs & Troubleshooting
FAQs
- What are Cap-0 and Cap-1?
- Can Faustovirus Capping Enzyme (NEB #M2081) use cap analogs in a capping reaction?
- Can Faustovirus Capping Enzyme (NEB #M2081) use GTP analogs in a capping reaction?
- Is Faustovirus Capping Enzyme sensitive to secondary structure?
- Is Faustovirus Capping Enzyme active at temperatures other than 37°C?
Citations & Technical Literature
Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- M2081L_v1_10160699
- M2081S_v1_10160698
- M2081L_v1_10164285
- M2081S_v1_10164284
- M2081L_v1_10185134
- M2081S_v1_10185135
- M2081L_v1_10185380
- M2081L_v1_10189866
- M2081L_v1_10206086
- M2081L_v1_10216765
- M2081S_v1_10216764
- M2081L_v1_10230206
- M2081L_v1_10238194
- M2081S_v1_10235973
- M2081S_v1_10251727
- M2081L_v1_10264780
- M2081S_v2_10264781
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.Faustovirus Capping Enzyme
Capping Buffer
S-adenosylmethionine (SAM)
GTP
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Licenses
"GMP-grade" is a branding term NEB uses to describe reagents manufactured at NEB’s Rowley facility. The Rowley facility was designed to manufacture reagents under more rigorous infrastructure and process controls to achieve more stringent product specifications and customer requirements. Reagents manufactured at NEB’s Rowley facility are manufactured in compliance with ISO 9001 and ISO 13485 quality management system standards. However, at this time, NEB does not manufacture or sell products known as Active Pharmaceutical Ingredients (APIs), nor does NEB manufacture its products in compliance with all of the Current Good Manufacturing Practice regulations.The supporting documents available for this product can be downloaded below.