Product Class: Other

3´-O-Me-m7G(5')ppp(5')G RNA Cap Structure Analog

Product Introduction

For most RNAs the cap structure increases stability, decreases susceptibility to exonuclease degradation, and promotes the formation of mRNA initiation complexes.

  • Also known as Anti-Reverse Cap Analog (ARCA)
  • Produces 100% translatable capped transcripts
  • Can be used for co-transcriptional capping with T7 (NEB #M0251), SP6 (NEB #M0207) and T3 RNA Polymerases
  • Can be used for synthesis of m7G capped RNA for in vitro splicing assays
  • Can be used for synthesis of m7G capped RNA for transfection or microinjection
  • Supplied as a dried sodium salt
Catalog # Size Concentration
S1411S 1.0 µmol
S1411L 5.0 µmol

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Product Information

Description

Blocking of the 3´ -hydroxyl of m7G with 3´-0-Me assures that the capped transcripts are homogeneous. The 3´ - hydroxyl of the non-methylated G is the only 3´ - hydroxyl available for initiation (1).

The 5´terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level (2,3,4). For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation (5), and a decrease in the formation of the initiation complex of mRNAs for protein synthesis (5,6). Certain prokaryotic mRNAs containing a 5´ terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system (6). Also a cap requirement has been observed for splicing eukaryotic substrate RNAs (7).

A method using E. coli RNA Polymerase primed with m7G(5´)ppp(5´)G or m7G(5´)ppp(5´)A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas (8). Larger amounts of capped RNAs are produced by transcription systems using SP6 RNA Polymerase primed with m7G(5´)ppp(5´)G (7).

Sequence

3'-O-Me-7mG(5')ppp(5')G
This product is related to the following categories:

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Supplied As

Dried Sodium Salt

Suggested Working Concentration

10mM

Recommended Resuspension Volume

100 μl

Product Notes

  1. Addition of 100 µl of water gives approximately a 10 mM solution.

References

  1. Peng, Z.-H. et al. (2002). ORG.LETT.. 4(2), 161.
  2. Shatkin, A. J. (1978). Cell. 9, 645-653.
  3. Fillipowicz, W. (1978). FEBS Lett. 96, 1-11.
  4. Banerjee, A.K. (1980). Microbiol. Rev.. 44, 175-205.
  5. Miura, K. (1981). Adv. Biophys.. 14, 205-238.
  6. Shatkin, A. J. et al. (1977). Nucleic Acids. Res.. 4, 3065-3081.
  7. Konarska, M. M. et al. (1984). Cell. 38, 731-736.
  8. Contreas, R. et al. (1982). Nucleic Acids. Res.. 10, 6353-6363.
  9. Paterson, B. M. and Rosenberg, M. (1979). Nature. 279, 696-701.

Protocols, Manuals & Usage

Protocols

  1. Capped RNA Synthesis (E2050)

Tools & Resources

Selection Charts

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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