Product Class: Other

RNase Inhibitor, Murine
recombinant

GMP-grade reagent also available. Learn more.

Product Introduction

RNase Inhibitor, Murine, specifically inhibits RNases A, B and C.

  • Improved resistance to oxidation, compared to human/porcine RNase inhibitor
  • Ideal for reactions where low DTT concentrations are required (e.g., Real-time PCR)
  • Isolated from a recombinant source
  • Tested for the absence of DNases and RNases
  • Getting ready to scale up RNA synthesis? Download our new technical note “Scaling of High-Yield In vitro Transcription Reactions for Linear Increase of RNA Production” for a generalized set of recommendations for synthesizing high yields of RNA.
Catalog # Size Concentration
M0314S 3000.0 units 40000 units/ml
M0314L 15000.0 units 40000 units/ml

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Product Information

Description

RNase Inhibitor, Murine is a 50 kDa recombinant protein of murine origin. The inhibitor specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).

Recombinant murine RNase inhibitor does not contain the pair of cysteines identified in the human version that is very sensitive to oxidation, which causes inactivation of the inhibitor (1). As a result, RNase Inhibitor, Murine has significantly improved resistance to oxidation compared to the human/porcine RNase inhibitors, and is stable at low DTT concentrations (less than 1 mM). This makes it ideal for reactions where high concentration DTT is adverse to the reaction (eg. Real-time RT-PCR).

Product Source

An E. coli strain that carries the Ribonuclease Inhibitor gene from mouse.
This product is related to the following categories:
RNase Inhibitors,
PURExpress,
Cell-Free Protein Expression Products,
cDNA Synthesis & Reverse Transcriptases Products,
RNA Synthesis In vitro Transcription (IVT),
Protein Expression Products,
This product can be used in the following applications:
NEBExpress® Cell-free E. coli Protein Synthesis System,
PURExpress,
RNA Cloning,
Cell-Free Protein Expression,
Protein Expression,
PCR

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Unit Definition

One unit is defined as the amount of Murine RNase Inhibitor required to inhibit the activity of 5 ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2', 3'-cyclic monophosphate by RNase A.

Storage Buffer

20 mM HEPES-KOH
50 mM KCl
8 mM DTT
50% Glycerol
pH 7.6 @ 25°C

Application Features

  • RT-PCR
  • cDNA synthesis
  • In vitro transcription/translation
  • Enzymatic RNA labeling reaction
  • Other applications where the integrity of RNA is important

Product Notes

  1. Since ribonucleases typically retain activity under denaturing conditions, care must be taken to avoid denaturing RNase Inhibitor molecules which have complexed with a ribonuclease. To prevent the release of active ribonuclease, temperatures greater than 50°C and high concentrations of urea or other denaturing agents should be avoided.
  2. The recommended concentration of RNase Inhibitor in a reaction is 1 unit/μl. During assembly of a reaction, RNase Inhibitor should be added before other components that are a possible source of RNase contamination (i.e. enzymes, plasmid from a mini prep.)

References

  1. Kim, B.M. et al. Protein Science. 8, 430-434.

Protocols, Manuals & Usage

Protocols

  1. Protocol for Avoiding Rnase Contamination using Murine Rnase Inhibitor (M0314)
  2. Poly(A) Tailing of RNA using E. coli Poly(A) Polymerase (NEB# M0276)

Application Notes

FAQs & Troubleshooting

Tech Tips

The inhibitor is a 50KD protein. It will be inactivated in conditions that will denature proteins.
We recommend using the inhibitor at 1U/ul in your reaction.
The inhibitor should be added in a reaction before other components that are a possible source of RNase contamination.
The inhibitor should be used below 50°C.

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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