Product Class: Other

LongAmp® Taq DNA Polymerase
NEBU cloned at NEB recombinant No pcr 5

Product Introduction

Exceptional performance with long amplicons

  • Optimized enzyme blend for amplification up to 30 kb
  • 2X higher fidelity than Taq DNA Polymerase
  • Hot start and master mix versions available
Catalog # Size Concentration
M0323S 500.0 units 2500 units/ml
M0323L 2500.0 units 2500 units/ml

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Product Information

Description

LongAmp® Taq DNA Polymerase is a unique blend of Taq and Deep Vent® DNA Polymerases. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). LongAmp Taq DNA Polymerase offers two fold higher fidelity than Taq DNA Polymerase alone. A wide range of PCR products can be generated, up to 30 kb from lambda DNA or from human genomic DNA.

Amplification of specific sequences from human genomic DNA using LongAmp Taq DNA Polymerase. Amplicon sizes are indicated below the gel. Marker M is the NEB 1 kb DNA Ladder (NEB #N3232).

Product Source

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent® DNA Polymerase gene from Pyrococcus species GB-D.
This product is related to the following categories:
LongAmp® DNA Polymerases Products,
This product can be used in the following applications:
Colony PCR,
Long Range PCR,
Specialty PCR,
Routine PCR,
PCR

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • M0323S     -20    
      LongAmp® Taq DNA Polymerase M0323SVIAL -20 1 x 0.2 ml 2,500 units/ml
      LongAmp® Taq Reaction Buffer Pack B0323SVIAL -20 1 x 1.5 ml 5 X
  • M0323L     -20    
      LongAmp® Taq DNA Polymerase M0323LVIAL -20 1 x 1 ml 2,500 units/ml
      LongAmp® Taq Reaction Buffer Pack B0323SVIAL -20 4 x 1.5 ml 5 X

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.

Reaction Conditions

1X LongAmp® Taq Reaction Buffer Pack

1X LongAmp® Taq Reaction Buffer Pack
60 mM Tris-SO4
20 mM (NH4)2SO4
2 mM MgSO4
3% Glycerol
0.06% IGEPAL® CA-630
0.05% Tween® 20
(pH 9.1 @ 25°C)

Storage Buffer

10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
0.5% Tween® 20
0.5% IGEPAL® CA-630
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

No

5' - 3' Exonuclease

Yes

3' - 5' Exonuclease

Yes

Strand Displacement

+

Resulting Ends

  • Mix of blunt and Single-base 3´ Overhangs

Unit Assay Conditions

1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA. 

Application Features

  • Long Range PCR
  • Colony PCR

Product Notes

  1. 5'→3' flap endonuclease destroys displaced strand.

References

  1. Barnes, W.M. (1994). Proc. Natl. Acad. Sci. 91, 2216-2220.
  2. Saiki R.K. et al. (1985). Science. 230, 1350-1354.
  3. Powell, L.M. et al. (1987). Cell. 50, 831-840.
  4. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
  5. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res. 18, 7465.

Protocols, Manuals & Usage

Protocols

  1. PCR Protocol for LongAmp® Taq DNA Polymerase (M0323)

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Is Monarch-purified gDNA suitable for long-range PCR?
  2. What ends will my PCR products have?
  3. How should I determine the appropriate annealing temperature for my reaction?
  4. What are the properties of this polymerase (fidelity, product ends, max amplicon, modified base incorporation, etc.)?
  5. My results are not as expected. Where can I find troubleshooting help?

Troubleshooting

Tech Tips

Using an extension temperature of 65°C is recommended in LongAmp Taq reactions.

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Trademarks

 
LONGAMP® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.
DEEP VENT® is a trademark of New England Biolabs, Inc.

IGEPAL® is a registered trademark of Rhodia Operations.
TWEEN® is a registered trademark of Uniqema Americas LLC.

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