Product Class: Kit

LongAmp® Taq PCR Kit

Product Introduction

Exceptional performance with long amplicons

  • Optimized enzyme blend for amplification up to 30 kb from low complexity templates and difficult templates, such has human genomic DNA
  • 2X higher fidelity than Taq
  • Kit contains 5X Reaction Buffer for optimal performance
Catalog # Size Concentration
E5200S 100.0 reactions (50.0 µl)

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Product Information

Description

The LongAmp Taq PCR Kit features LongAmp Taq DNA Polymerase, which is a unique blend of Taq and Deep Vent® DNA Polymerases. The 3´→5´ exonuclease activity of Deep Vent® Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). LongAmp Taq DNA Polymerase offers two fold higher fidelity than Taq Polymerase alone. The LongAmp Taq PCR Kit is supplied with LongAmp Taq Reaction Buffer, which is optimized to give highest PCR yields up to 30 kb. The kit can generate a wide range of PCR products, up to 30 kb from plasmid or from human genomic DNA.


Amplification of specific sequences from human genomic DNA using LongAmp Taq DNA Polymerase. Amplicon sizes are indicated below the gel. Marker M is NEB 1 kb DNA Ladder (NEB #N3232).
This product is related to the following categories:
LongAmp® DNA Polymerases Products,
This product can be used in the following applications:
Long Range PCR,
Specialty PCR,
Routine PCR,
PCR

Kit Components

Kit Components

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Application Features

  • Long Range PCR
  • Colony PCR

References

  1. Barnes, W.M. (1994). Proc. Natl. Acad. Sci. 91, 2216-2220.
  2. Saiki R.K. et al. (1985). Science. 230, 1350-1354.
  3. Powell, L.M. et al. (1987). Cell. 50, 831-840.
  4. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
  5. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res. 18, 7465.

Protocols, Manuals & Usage

Protocols

  1. Protocol for LongAmp™ Taq PCR Kit

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. What is the recommended enzyme amount when using LongAmp™ Taq DNA Polymerase?
  2. Can the extension step be carried out at 72°C when using LongAmp™ Taq DNA Polymerase?
  3. What is the extension rate when using LongAmp™ Taq DNA Polymerase?
  4. Why is the product a smear when visualized on an agarose gel?
  5. Can the LongAmp™ kit be used to amplify GC-rich amplicons?
  6. What type of DNA ends result from a primer extension reaction or a PCR using LongAmp™ Taq DNA Polymerase?
  7. What is the fidelity of the LongAmp™ Taq DNA Polymerase compared to Taq DNA Polymerase?
  8. Can NEBuilder® HiFi DNA Assembly master mix remove both 3′ and 5′ end mismatches? 

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

Purchase of this product provides the purchaser with a non-exclusive license to use LongAmp® Taq PCR Kit for research purposes only. Commercial use of this product may require a license from New England Biolabs, Inc. under U.S. Patent No. 5,352,778.

Trademarks

LONGAMP® and NEW ENGLAND BIOLABS® are registered trademarks of New England Biolabs, Inc.
DEEP VENT® is a trademark of New England Biolabs, Inc.

IGEPAL® is a registered trademark of Rhodia Operations.
TWEEN® is a registered trademark of Uniqema Americas LLC.

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