Quick-Load® 1 kb Extend DNA Ladder
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
N3239S |
Quick-Load 1 kb Extend DNA Ladder |
125 gel assays ( 50 µg/ml ) | - | Unavailable in your region |
Quick-Load® 1 kb Extend DNA Ladder
Product Introduction
- Size range: 0.5 kb to 48.5 kb
- Convenient, ready-to-load format
- Formulated with bromophenol blue dye
- Easy to identify reference bands
- Suitable for 125 gel lanes
Catalog # | Size | Concentration |
---|---|---|
N3239S | 125.0 gel lanes (1.25 ml) | 50 µg/ml |
- Product Information
- Protocols, Manuals & Usage
- FAQs & Troubleshooting
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Product Information
Description
The Quick-Load 1 kb Extend DNA ladder is a pre-mixed, ready-to-load molecular weight DNA marker containing bromophenol blue dye as a tracking dye.
The DNA Ladder consists of proprietary plasmids and DNA molecules which are digested to completion with appropriate restriction enzymes to yield 13 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 0.5 to 48.5 kilobases. The 3 kb fragment has increased intensity to serve as reference band.
- Recommended gel and migration conditions: 0.5% 1X TBE agarose, 5V/cm (distance between electrodes), for 90 to 105 minutes
- This product is related to the following categories:
- DNA Markers & Ladders Products
- This product can be used in the following applications:
- DNA Analysis
Reagents Supplied
Reagents Supplied
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration | |
---|---|---|---|---|---|---|
Properties & Usage
Bases
Fragment | Mass (ng) | bp |
---|---|---|
1 | 30 | 48,502 |
3 | 45 | 15,000 |
4 | 33 | 10,002 |
5 | 33 | 8,001 |
6 | 40 | 6,001 |
7 | 33 | 5,001 |
8 | 26 | 4,001 |
9 | 100 | 3,001 |
10 | 37 | 2,000 |
11 | 27 | 1,500 |
12 | 33 | 1,000 |
2a | 15 | 20,066 |
2b | 15 | 19,871 |
13a | 18 | 517 |
13b | 17 | 500 |
Preparation
The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.Effective Size Range
500bp to 48,502bpStorage Buffer
2.5% Ficoll®-400
11 mM EDTA
3.3 mM Tris-HCl
0.017% SDS
0.015% bromophenol blue
pH 8 @ 25°C
Related Products
Companion Products
- Quick-Load® 1 kb DNA Ladder
- λ DNA-HindIII Digest
- Monarch® Plasmid Miniprep Kit
- Monarch® DNA Gel Extraction Kit
- Monarch® PCR & DNA Cleanup Kit (5 μg)
Materials Sold Separately
Product Notes
- Quick-Load 1 kb Extend DNA Ladder is stable for at least 6 months at 25°C.
Store at 4°C. Do not store at -20°C. - Usage Recommendation: We recommend loading 10 μl (0.5 μg) of Quick-Load 1 kb Extend DNA Ladder per gel lane. For optimum separation of the highest molecular weight DNA fragments, run on a 0.6% (1X TAE or 1X TBE) agarose gel at 3.5 V/cm (distance between electrodes) for 3 hours.
In general, it is recommended to load 1 ul per mm of gel lane.The Quick-Load 1 kb Extend DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
References
- Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Cold Spring Harbor: Cold Spring Harbor Laboratory Press.(Ed.), Molecular Cloning: A Laboratory Manual. pp. 10.51-10.67.
Protocols, Manuals & Usage
Protocols
FAQs & Troubleshooting
FAQs
Citations & Technical Literature
Citations
Additional Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- N3239S_v1_0021411
- N3239S_v1_0031504
- N3239S_v1_0031508
- N3239S_v1_0031512
- N3239S_v1_0041602
- N3239S_v1_0041608
- N3239S_v1_0051612
- N3239S_v1_0051704
- N3239S_v1_0051711
- N3239S_v1_0061712
- N3239S_v1_0061804
- N3239S_v1_10021327
- N3239S_v1_10034202
- N3239S_v1_10045373
- N3239S_v1_10064807
- N3239S_v1_10081799
- N3239S_v1_10092192
- N3239S_v1_10100148
- N3239S_v1_10123742
- N3239S_v1_10151850
- N3239S_v1_10175988
- N3239S_v1_10204919
- N3239S_v1_10240121
- N3239S_v1_10268612
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.Quick-Load® 1 kb Extend DNA Ladder
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Trademarks
QUICK-LOAD® is a registered trademark of New England Biolabs, Inc.FICOLL® is a registered trademark of GE Healthcare Bio-Sciences AB.
Other Products You May Be Interested In
The supporting documents available for this product can be downloaded below.